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G-CSF ELISA Kit

CSF3 Reaktivität: Maus Colorimetric Sandwich ELISA 0.5-150 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN1979761
  • Target Alle G-CSF (CSF3) ELISA Kits anzeigen
    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
    Reaktivität
    • 8
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.5-150 pg/mL
    Untere Nachweisgrenze
    0.5 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Mouse GCSF ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Serum, Plasma, Cell Culture Supernatant
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
    Sensitivität
    < 0.5 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples20 - 100 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Aufbereitung der Reagenzien
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
      2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants. Suggested dilution for normal serum/plasma: 20-100 fold. Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
      3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
      4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium) into Item C vial to prepare a 50 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 2 µL G-CSF standard from the vial of Item C, into a tube with 664.7 µL Assay Diluent A or 1x Assay Diluent B to prepare a 150 pg/mL stock standard solution. Pipette 400 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 200 µL 200myl 200 µL 200 µL 2 µL standard +664.7myl 200 µL 150 50 16.67 5.56 1.85 0.62 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
      5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
      6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 65-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
      7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 80-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 150 µL of HRP-Streptavidin concentrate into a tube with 12 ml 1x Assay Diluent B to prepare a final 80 fold diluted HRP-Streptavidin solution. Mix well.
    Testdurchführung
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
      2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
      3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
      4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
      5. Discard the solution. Repeat the wash as in step
      6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
      7. Discard the solution. Repeat the wash as in step
      8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
      9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
    Ergebnisberechnung

    Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
    Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A G-CSF concentration (pg/mL) 0.1 1 10 100 1000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B G-CSF concentration (pg/mL) 0.1 1 10 100 1000 O D =4 50 n m 0.01 0.1 1 10
    Sensitivity: The minimum detectable dose of G-CSF is typically less than 0.5 pg/mL.
    Recovery: Recovery was determined by spiking various levels of mouse G-CSF into mouse serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 95.53 83-104 Plasma 94.28 82-102 Cell culture media 97.52 84-104
    Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 94 95 93 Range ( %) 83-103 84-103 82-102 1:4 Average % of Expected 97 96 94 Range ( %) 85-105 84-104 83-102
    Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %

    Testpräzision
    Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Li, Wang, Yi, Jia, Bai, Peng, Yu, Xiong, Xing, Shan, Yang, Dong, Cong: "Succinate ester derivative of δ-tocopherol enhances the protective effects against 60Co γ-ray-induced hematopoietic injury through granulocyte colony-stimulating factor induction in mice." in: Scientific reports, Vol. 7, pp. 40380, (2018) (PubMed).

    Hollmén, Karaman, Schwager, Lisibach, Christiansen, Maksimow, Varga, Jalkanen, Detmar: "G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer." in: Oncoimmunology, Vol. 5, Issue 3, pp. e1115177, (2016) (PubMed).

    Zhang, Ren, Tang, Wang, Liu, Zhang, Li, Liu, Zhao, He: "Vascular normalization induced by sinomenine hydrochloride results in suppressed mammary tumor growth and metastasis." in: Scientific reports, Vol. 5, pp. 8888, (2015) (PubMed).

    Pathak, Shao, Ghosh, Zhou, Boerma, Weiler, Hauer-Jensen: "Thrombomodulin contributes to gamma tocotrienol-mediated lethality protection and hematopoietic cell recovery in irradiated mice." in: PLoS ONE, Vol. 10, Issue 4, pp. e0122511, (2015) (PubMed).

    Andzinski, Wu, Lienenklaus, Kröger, Weiss, Jablonska: "Delayed apoptosis of tumor associated neutrophils in the absence of endogenous IFN-β." in: International journal of cancer, Vol. 136, Issue 3, pp. 572-83, (2015) (PubMed).

    Xu, Shao, Wang, Zhou, Tang, Lu, Xiong: "Role of Interleukin-17 in defense against pseudomonas aeruginosa infection in lungs." in: International journal of clinical and experimental medicine, Vol. 7, Issue 4, pp. 809-16, (2014) (PubMed).

    Mishra, Patel, Bansal, Kumar: "Semiquinone glucoside derivative provides protection against ?-radiation by modulation of immune response in murine model." in: Environmental toxicology, (2014) (PubMed).

    Fulzele, Krause, Panaroni, Saini, Barry, Liu, Lotinun, Baron, Bonewald, Feng, Chen, Weinstein, Wu, Kronenberg, Scadden, Divieti Pajevic: "Myelopoiesis is regulated by osteocytes through Gs?-dependent signaling." in: Blood, Vol. 121, Issue 6, pp. 930-9, (2013) (PubMed).

    Fortin, Benabdallah, Palacio, Carbonneau, Le, Haddad, Beauséjour: "A soluble granulocyte colony stimulating factor decoy receptor as a novel tool to increase hematopoietic cell homing and reconstitution in mice." in: Stem cells and development, Vol. 22, Issue 6, pp. 975-84, (2013) (PubMed).

    Lee, Wolstein, Pudasaini, Plotkin: "INK4a deletion results in improved kidney regeneration and decreased capillary rarefaction after ischemia-reperfusion injury." in: American journal of physiology. Renal physiology, Vol. 302, Issue 1, pp. F183-91, (2011) (PubMed).

    Shukla, Kumar, Mishra, Chaudhari, Munjal, Tripathi, Raisuddin, Paul: "Carryover of cigarette smoke effects on hematopoietic cytokines to F1 mouse litters." in: Molecular immunology, Vol. 48, Issue 15-16, pp. 1809-17, (2011) (PubMed).

    Asano, Nishizawa, Nagata: "Three individual regulatory elements of the promoter positively activate the transcription of the murine gene encoding granulocyte colony-stimulating factor." in: Gene, Vol. 107, Issue 2, pp. 241-6, (1992) (PubMed).

    Barber, Crosier, Gillis, Watson: "Human granulocyte-macrophage progenitors and their sensitivity to cytotoxins: analysis by limiting dilution." in: Blood, Vol. 70, Issue 6, pp. 1773-6, (1988) (PubMed).

    Begley, Metcalf, Nicola: "Primary human myeloid leukemia cells: comparative responsiveness to proliferative stimulation by GM-CSF or G-CSF and membrane expression of CSF receptors." in: Leukemia, Vol. 1, Issue 1, pp. 1-8, (1987) (PubMed).

  • Target Alle G-CSF (CSF3) ELISA Kits anzeigen
    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
    Andere Bezeichnung
    G-CSF/ CSF3 (CSF3 Produkte)
    Synonyme
    CSF3 ELISA Kit, G-CSF ELISA Kit, gcsf ELISA Kit, Csfg ELISA Kit, MGI-IG ELISA Kit, C17orf33 ELISA Kit, CSF3OS ELISA Kit, GCSF ELISA Kit, Gcsf ELISA Kit, colony stimulating factor 3 ELISA Kit, colony stimulating factor 3 (granulocyte) a ELISA Kit, colony stimulating factor 3 (granulocyte) ELISA Kit, CSF3 ELISA Kit, csf3a ELISA Kit, Csf3 ELISA Kit
    Hintergrund
    G-CSF is an O-glycosylated glycoprotein, which is secreted by monocytes, macrophages and neutrophils. G-CSF is also produced by stromal cells, fibroblasts, and endothelial cells. Epithelial carcinomas, acute myeloid leukemia cells and various tumor cell lines, also express this factor. G-CSF stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage. It is a mitogen for some human myeloid leukemia cells and also for some carcinoma cell lines. In vitro G-CSF enhances the antibody-dependent cellular cytotoxicity of granulocytes against tumor cells. The Mouse G-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse G-CSF in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for mouse G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
    Gen-ID
    12985
    UniProt
    P09920
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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