Mdm4 / HdmX-Trap

Details zu Produkt Nr. ABIN1889486, Anbieter: Anmelden zum Anzeigen
Antigen
  • mdmx
  • HDMX
  • MDMX
  • MRP1
  • wu:fa09h09
  • wu:fi33d10
  • 4933417N07Rik
  • AA414968
  • AL023055
  • AU018793
  • AU021806
  • C85810
  • Mdmx
  • MDM4, p53 regulator S homeolog
  • MDM4, p53 regulator
  • transformed mouse 3T3 cell double minute 4
  • mdm4.S
  • MDM4
  • Mdm4
  • mdm4
Epitop
AA 1-129
Reaktivität
Human
4
2
2
Wirt
Camelid (Camelidae)
Applikation
Affinity Measurement (AM), Chromatin Immunoprecipitation (ChIP), Enzyme Activity Assay (EAA), Immunoprecipitation (IP), Mass Spectrometry (MS), Protein Complex Immunoprecipitation (Co-IP), Pull-Down Assay (Pull-Down), Purification (Purif)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Verwendungszweck The Mdm4/ HdmX-Trap is a high quality Mdm4/ HdmX-binding protein for biochemical analysis of Mdm4/ HdmX and interacting partners.
Proben Cell Extracts
Spezifität Species-Reactivity: tested on human, mouse, hamster
Produktmerkmale Mdm4/ HdmX-Trap is excellent for fast and efficient one-step isolation of Mdm4/ HdmX and its interacting factors from cellular extract. Isolated Mdm4/ HdmX protein may be used further for immunoblot analysis or mass spectrometry.
Mdm4/ HdmX-Trap utilizes small recombinant alpaca antibody fragments covalently coupled to the surface of agarose beads.
Reinigung Purifed protein
Bestandteile Mdm4/ HdmX-Trap uncoupled
Benötigtes Material Lysis buffer (CoIP), 10x RIPA buffer, Dilution buffer, Wash buffer, Elution buffer
Andere Bezeichnung Mdm4 (MDM4 ELISA Kit Abstract)
Hintergrund Double minute 4 protein (Mdm4/ HdmX/ MdmX) plays a role in cell cylce control and apoptosis by inhibition of p53 and p73.
Kommentare

Use this Mdm4/HdmX-Trap to couple it to your own immobilizing matrix.
Note that binding of Mdm4/ HdmX-Trap interferes with binding of p53 to Mdm4/ HdmX.

Testdauer 1.5 h
Protokoll
  • Robust and versatile tool for biochemical analyses of Mdm4/ HdmX proteins
  • Short incubation times (5 - 30 min)
  • Quantitative isolation of fusion proteins and transiently bound factors from cell extracts or organelles
  • Low unspecific binding
  • No contaminating heavy and light chains of conventional antibodies
  • Applicable in Chromatin Immunoprecipitation (ChIP)
Probennahme Harvest cells:
For one immunoprecipitation reaction the use of 10^6 - 10^7 mammalian cells (approx. one 10 cm dish) is recommended. To harvest adherent cells, aspirate growth medium, add 1 ml ice-cold PBS to cells and scrape cells from dish. Transfer cells to a pre-cooled tube, spin at 500 g for 3 min at +4°C and discard supernatant. Wash cell pellet twice with ice-cold PBS, gently resuspending the cells.

Lyse cells
  • 1. Resuspend cell pellet in 200 µL ice-cold lysis buffer by pipetting or using a syringe.
    note: Supplement lysis buffer with protease inhibitors and 1 mM PMSF (not included). optional for nuclear/chromatin proteins: Use RIPA buffer supplemented with 1 mg/mL DNase, 2.5 mM MgCl2, protease inhibitors and 1 mM PMSF (not included).
  • 2. Place the tube on ice for 30 min with extensively pipetting every 10 min.
  • 3. Centrifuge cell lysate at 20.000x g for 10 min at +4°C. Transfer lysate to a pre-cooled tube. Add 300 µl dilution buffer to lysate. Discard pellet.
    note: At this point cell lysate may be put at -80°C for long-term storage. optional: Add 1 mM PMSF and protease inhibitors (not included) to dilution buffer

We recommend that during incubation with the beads the final concentration of detergents does not exceed 0.2% to avoid unspecific binding to the matrix. If required, use more dilution buffer to dilute the supernatant accordingly.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer 1 x PBS, 0.01 % Sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Do not freeze.
Lagerung 4 °C
Haltbarkeit 12 months
Bilder des Herstellers
Immunoprecipitation (IP) image for Mdm4 / HdmX-Trap (ABIN1889486) Pulldown of Mdm4/HdmX using the Mdm4/HdmX-Trap:Immunoprecipitation (IP) of Mdm4/HdmX ...
Haben Sie etwas anderes gesucht?