IL1R2 ELISA Kit

Produktnummer ABIN1889297

Produktdetails IL1R2 ELISA Kit

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Bindungsspezifität: AA 14-343

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL1R2

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: Expression system for standard: sf21
Immunogen sequence: F14-E343

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: F14-E343

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the interleukin-1 receptor family.

Plattentyp: Pre-coated

Protokoll: human IL1R2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL1R2 has been precoated onto 96-well plates. Standards(sf21, F14-E343) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1R2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1R2 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL1R2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL1R2 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 85, Standard deviation: 3.91, CV(%): 4.6
• Sample 2: n=16, Mean(pg/ml): 392, Standard deviation: 12.2, CV(%): 3.1
• Sample 3: n=16, Mean(pg/ml): 750, Standard deviation: 28.5, CV(%): 3.8,
• Sample 1: n=24, Mean(pg/ml): 96, Standard deviation: 5.95, CV(%): 6.2
• Sample 2: n=24, Mean(pg/ml): 414, Standard deviation: 22.77, CV(%): 5.5
• Sample 3: n=24, Mean(pg/ml): 763, Standard deviation: 38.9, CV(%): 5.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu IL1R2

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Andere Bezeichnung: IL1R2 (IL1R2 Produkte)

Synonyme: CD121b ELISA Kit, CDw121b ELISA Kit, IL-1R-2 ELISA Kit, IL-1RT-2 ELISA Kit, IL-1RT2 ELISA Kit, IL1R2c ELISA Kit, IL1RB ELISA Kit, Il1r-2 ELISA Kit, IL-1R2 ELISA Kit, interleukin 1 receptor type 2 ELISA Kit, interleukin 1 receptor, type II ELISA Kit, interleukin-like ELISA Kit, IL1R2 ELISA Kit, Il1r2 ELISA Kit, KK34 ELISA Kit

Hintergrund:

Protein Function: Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity, this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. .

Background: Interleukin 1 receptor, type II(IL1R2) also known as CD121b(Cluster of Differentiation 121b) is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. It is mapped to 2q11.2. IL1R2 binds interleukin alpha(IL1A), interleukin beta(IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. The secreted IL1R2 recruits secreted IL1RAP with high affinity, and this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.

Synonyms: Interleukin-1 receptor type 2,IL-1R-2,IL-1RT-2,IL-1RT2,CD121 antigen-like family member B,CDw121b,IL-1 type II receptor,Interleukin-1 receptor beta,IL-1R-beta,Interleukin-1 receptor type II,CD121b,Interleukin-1 receptor type 2, membrane form,mIL-1R2,mIL-1RII,Interleukin-1 receptor type 2, soluble form,sIL-1R2,sIL-1RII,IL1R2,IL1RB,

Full Gene Name: Interleukin-1 receptor type 2

Cellular Localisation: Isoform Short: Secreted.

Gen-ID: 7850

UniProt: P27930

Pathways: NF-kappaB Signalweg