SensoLyte® Generic MMP Assay Kit

Produktnummer ABIN1882502

Produktdetails

Target: Matrix Metalloproteinase

Nachweismethode: Colorimetric

Applikation: Enzyme Activity Assay (EAA)

Marke: SensoLyte®

Produktmerkmale: The SensoLyte® Generic MMP Assay Kit can be used to detect the activity of a variety of MMPs, including MMP-1, 2, 3, 7, 8, 9, 12, 13, and 14 or for high throughput screening of MMPs' inducers and inhibitors. The substrate is a thiopeptolide that is cleaved by the MMPs, releasing a sulfhydryl group, which can be detected with a color-developing thiol-reactive agent, 4,4'-dithiodipyridine or Ellman's Reagent at 412 nm using a microplate reader. The assays are performed in a convenient 96-well microplate format.

Anwendungsinformationen

Kommentare:

Enzymatic Activity Assay kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Aliquot Components E and F as needed to avoid freeze-thaw cycles. Protect from light.

Lagerung: -20 °C

Informationen zur Lagerung: Store all kit components at -20 °C. Store Component C at 4 °C in the dark. Component G can be stored at room temperature for convenience.

Referenzen

Li, Mittelstein, Lee, Fang, Majumdar, Tintut, Demer, Hsiai: "A dynamic model of calcific nodule destabilization in response to monocyte- and oxidized lipid-induced matrix metalloproteinases." in: American journal of physiology. Cell physiology, Vol. 302, Issue 4, pp. C658-65, (2012) (PubMed).

Spolidoro, Putignano, Munafò, Maffei, Pizzorusso: "Inhibition of matrix metalloproteinases prevents the potentiation of nondeprived-eye responses after monocular deprivation in juvenile rats." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 22, Issue 3, pp. 725-34, (2012) (PubMed).

Tezvergil-Mutluay, Mutluay, Agee, Seseogullari-Dirihan, Hoshika, Cadenaro, Breschi, Vallittu, Tay, Pashley: "Carbodiimide cross-linking inactivates soluble and matrix-bound MMPs, in vitro." in: Journal of dental research, Vol. 91, Issue 2, pp. 192-6, (2012) (PubMed).

Tezvergil-Mutluay, Agee, Uchiyama, Imazato, Mutluay, Cadenaro, Breschi, Nishitani, Tay, Pashley: "The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs." in: Journal of dental research, Vol. 90, Issue 4, pp. 535-40, (2011) (PubMed).

Thompson, Agee, Sidow, McNally, Lindsey, Borke, Elsalanty, Tay, Pashley: "Inhibition of endogenous dentin matrix metalloproteinases by ethylenediaminetetraacetic acid." in: Journal of endodontics, Vol. 38, Issue 1, pp. 62-5, (2011) (PubMed).

Tezvergil-Mutluay, Agee, Hoshika, Tay, Pashley: "The inhibitory effect of polyvinylphosphonic acid on functional matrix metalloproteinase activities in human demineralized dentin." in: Acta biomaterialia, Vol. 6, Issue 10, pp. 4136-42, (2010) (PubMed).

Antigendetails

Target: Matrix Metalloproteinase

Andere Bezeichnung: MMP

Hintergrund: Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known.