TFPI ELISA Kit

Produktnummer ABIN1672813

Produktdetails TFPI ELISA Kit

Target: TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))

Bindungsspezifität: AA 29-282

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TFPI

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: D29-K282

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: D29-K282

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Contains 3 BPTI/Kunitz inhibitor domains.

Tissue Specificity: Mostly in endothelial cells.

Plattentyp: Pre-coated

Protokoll: human TFPI ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFPI has been precoated onto 96-well plates. Standards(NSO, D29-K282) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFPI is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TFPI amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TFPI standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TFPI standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 313, Standard deviation: 16.3, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 572, Standard deviation: 24.6, CV(%): 4.3
• Sample 3: n=16, Mean(pg/ml): 1215, Standard deviation: 47.4, CV(%): 3.9,
• Sample 1: n=24, Mean(pg/ml): 327, Standard deviation: 21.6, CV(%): 6.6
• Sample 2: n=24, Mean(pg/ml): 588, Standard deviation: 34.1, CV(%): 5.8
• Sample 3: n=24, Mean(pg/ml): 1279, Standard deviation: 79.3, CV(%): 6.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für TFPI ELISA Kit (ABIN1672813)

Xu, Qin, Zhou, Tu, Bi, Li, Fan, Zhang: "Tissue factor pathway inhibitor-2 inhibits the growth and invasion of hepatocellular carcinoma cells and is inactivated in human hepatocellular carcinoma." in: Oncology letters, Vol. 2, Issue 5, pp. 779-783, (2012) (PubMed).

Details zu TFPI

Target: TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))

Andere Bezeichnung: TFPI (TFPI Produkte)

Synonyme: EPI ELISA Kit, LACI ELISA Kit, TFI ELISA Kit, TFPI1 ELISA Kit, A630013F22Rik ELISA Kit, AW552122 ELISA Kit, TFPI ELISA Kit, si:dz44f8.1 ELISA Kit, tfpib ELISA Kit, wu:fb92g01 ELISA Kit, zgc:152844 ELISA Kit, zgc:158592 ELISA Kit, tissue factor pathway inhibitor ELISA Kit, tissue factor pathway inhibitor a ELISA Kit, TFPI ELISA Kit, Tfpi ELISA Kit, tfpia ELISA Kit

Hintergrund:

Protein Function: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

Background: TFPI(Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa(Xa) and Thrombin(Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus.TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.

Synonyms: Tissue factor pathway inhibitor,TFPI,Extrinsic pathway inhibitor,EPI,Lipoprotein-associated coagulation inhibitor,LACI,TFPI,LACI, TFPI1,

Full Gene Name: Tissue factor pathway inhibitor

Cellular Localisation: Isoform Alpha: Secreted.

Gen-ID: 7035

UniProt: P10646