Lamin A/C ELISA Kit

Produktnummer ABIN1571047

Produktdetails Lamin A/C ELISA Kit

Target: Lamin A/C (LMNA)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Verwendungszweck: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of LMNA in human tissue homogenates and other biological fluids.

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of this index.

Kreuzreaktivität (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensitivität: 0.054 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Benötigtes Material:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Testdurchführung:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Haltbarkeit: 12 months

Details zu Lamin A/C

Target: Lamin A/C (LMNA)

Andere Bezeichnung: LMNA (LMNA Produkte)

Synonyme: CDCD1 ELISA Kit, CDDC ELISA Kit, CMD1A ELISA Kit, CMT2B1 ELISA Kit, EMD2 ELISA Kit, FPL ELISA Kit, FPLD ELISA Kit, FPLD2 ELISA Kit, HGPS ELISA Kit, IDC ELISA Kit, LDP1 ELISA Kit, LFP ELISA Kit, LGMD1B ELISA Kit, LMN1 ELISA Kit, LMNC ELISA Kit, LMNL1 ELISA Kit, PRO1 ELISA Kit, Dhe ELISA Kit, 2459 ELISA Kit, 74/76 ELISA Kit, CG6944 ELISA Kit, D5 ELISA Kit, DM[[O]] ELISA Kit, Dm ELISA Kit, Dm(0) ELISA Kit, Dm0 ELISA Kit, Dm1 ELISA Kit, Dm2 ELISA Kit, DmLamin ELISA Kit, DmO ELISA Kit, Dm[[0]] ELISA Kit, Dm[[1]] ELISA Kit, Dm[[2]] ELISA Kit, Dm[[mit]] ELISA Kit, Dm[[o]] ELISA Kit, Dmel\\CG6944 ELISA Kit, Dmo ELISA Kit, LAM ELISA Kit, Lam Dm[[0]] ELISA Kit, Lam(Dm0) ELISA Kit, LamDm[[0]] ELISA Kit, LamDm[[o]] ELISA Kit, Lam[[Dm0]] ELISA Kit, Lamin Dm[[0]] ELISA Kit, jf27 ELISA Kit, l(2)04643 ELISA Kit, l(2)25Ec ELISA Kit, l(2)gdh-7 ELISA Kit, l(2)gdh7 ELISA Kit, l(2)jf27 ELISA Kit, lam ELISA Kit, lamDm0 ELISA Kit, lamDm[[0]] ELISA Kit, lamin ELISA Kit, lamin Dm0 ELISA Kit, lamin Dm[[0]] ELISA Kit, misg ELISA Kit, nlam ELISA Kit, cb948 ELISA Kit, fk66d12 ELISA Kit, wu:fk66d12 ELISA Kit, lmna-A ELISA Kit, fpl ELISA Kit, idc ELISA Kit, lfp ELISA Kit, cddc ELISA Kit, emd2 ELISA Kit, fpld ELISA Kit, hgps ELISA Kit, ldp1 ELISA Kit, lmn1 ELISA Kit, lmnc ELISA Kit, pro1 ELISA Kit, cdcd1 ELISA Kit, cmd1a ELISA Kit, cmt2b1 ELISA Kit, lgmd1b ELISA Kit, Lamin-A ELISA Kit, lamin-L(III)-like ELISA Kit, lamin A/C ELISA Kit, lamin A ELISA Kit, Lamin ELISA Kit, lamin A/C L homeolog ELISA Kit, lamin-L(III)-like ELISA Kit, LMNA ELISA Kit, Lmna ELISA Kit, Lam ELISA Kit, lmna ELISA Kit, lmna.L ELISA Kit, LMINA ELISA Kit

Hintergrund: Alternative name: CDCD1, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, PRO1, Cardiomyopathy Dilated 1A, Limb Girdle Muscular Dystrophy 1B, Progeria 1

Gen-ID: 4000

UniProt: P02545

Pathways: Apoptose, Caspase Kaskade in der Apoptose, ER-Nucleus Signaling, Protein targeting to Nucleus