PEP Colorimetric/Fluorometric Assay Kit

Details zu Produkt Nr. ABIN1304362, Anbieter: Anmelden zum Anzeigen
  • PE
  • PEP
  • AI047692
  • AI450383
  • D10Wsu136e
  • Pop
  • rPop
  • im:7140031
  • zgc:110670
  • prolyl endopeptidase
  • Prolyl endopeptidase
  • RB12337
  • AM1_4884
  • ppce
  • PREP
  • Prep
  • prep
Untere Nachweisgrenze
1 µM
Biochemical Assay (BCA)
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Hersteller Produkt- Nr.
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Proben Plasma, Serum, Cell Lysate, Tissue Samples
Nachweismethode Fluorometric, Colorimetric
Produktmerkmale PEP Colorimetric,Fluorometric Assay Kit: Sensitive & Convenient Colorimetric and Fluorometric Assay to measure PEP levels.
Simple procedure, Fast and convenient, The assay is sensitive, stable and high-throughput adaptable.

Detection wavelength: 570 nm, Ex/Em 535/587 nm.
Bestandteile PEP Assay Buffer
PEP Probe (in DMSO)
PEP Converter
PEP Developer Mix
PEP Standard (1 μmole)
Andere Bezeichnung PEP (PREP ELISA Kit Abstract)
Hintergrund Phosphoenolpyruvate (PEP) is an important intermediate in carbohydrate metabolism. Containing a high-energy phosphate bond, PEP is involved in glycolysis and gluco-neogenesis and in the shikimate pathway as well as in carbon fixation in plants. Bacteria utilize PEP in the phosphor-transferase system to acquire sugars from the environment. In the glycolytic pathway, PEP is formed from 2-phosphoglycerate by enolase and generates ATP through the action of pyruvate kinase.
Applikationshinweise The detection limit is approximately 1 μM PEP in biological samples.

Further details regarding sample type: Cell and tissue lysates, urine, plasma, serum, and plant extracts

Protokoll In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (lambdamax = 570 nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, sensitive and reliable. The detection limit is approximately 1 µM PEP in biological samples.
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Protect from light. Product is photosensitive.
Lagerung -20 °C
Haltbarkeit 12 months
Bilder des Herstellers
 image for PEP Colorimetric/Fluorometric Assay Kit (ABIN1304362) PEP Colorimetric/Fluorometric Assay Kit
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