anti-Pferd IL21 Antikörper für ELISA

Recommended IL21 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Interleukin 21 (IL21) Antikörper
  • IL-21
  • Za11
  • interleukin 21
  • IL21
  • Il21
Epitop
AA 24-152
16
15
11
9
8
8
7
6
6
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Schwein, Pferd
203
98
28
7
4
2
2
1
1
Wirt
Kaninchen
171
69
31
9
4
Klonalität
Polyklonal
Konjugat
Dieser IL21 Antikörper ist unkonjugiert
24
12
12
10
10
8
6
4
4
4
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
192
131
49
40
30
23
14
13
10
7
6
6
4
4
4
3
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN964709
$ 583.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
4 ABIN1102475 ELISA WB Rabbit IgG AA 24-152 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Interleukin 21 (IL21) Antikörper
Epitop AA 24-152
(16), (15), (11), (9), (8), (8), (7), (6), (6), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Rind (Kuh), Schwein, Pferd
(203), (98), (28), (7), (4), (2), (2), (1), (1)
Wirt Kaninchen
(171), (69), (31), (9), (4)
Klonalität
Polyklonal
Konjugat Dieser IL21 Antikörper ist unkonjugiert
(24), (12), (12), (10), (10), (8), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation ELISA, Western Blotting (WB)
(192), (131), (49), (40), (30), (23), (14), (13), (10), (7), (6), (6), (4), (4), (4), (3), (2), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-IL21 Antikörper

Target Details IL21 Anwendungsinformationen Handhabung Bilder
Spezifität This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine IL-21 protein. A BLAST analysis was used to suggest cross-reactivity with IL-21 from bovine sources based on 100% homology with the immunizing sequence. Based on 92% homology, there is a chance of cross-reactivity to porcine IL- 21, 91% to horse IL-21, 89% to human and dog, 88% to macaque, 84% to platypus and Syrian hamster, 76-79% to mouse, 75% to rat, and 48% to chicken. Cross-reactivity with IL-21 from other sources has not been determined.
Kreuzreaktivität Schwein, Pferd
Produktmerkmale Interleukin-21 (IL-21) is a secreted, type-I cytokine with immunoregulatory activity. Human IL-21 shares the common gamma-chain with IL-2, IL-4, IL-7, IL-9, and IL-15 proteins but, in addition, binds to a unique IL-21Ralpha chain which triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Bovine and human IL-21 have pleiotropic functions and are mainly produced by activated T-cells in response to antigenic stimulation, but target a broad range of lymphoid and myeloid cells of the immune system (T cells, B cells, natural killer (NK) cells and dendritic cells). IL21 is therefore is able to regulate innate and acquired immune responses. The biological effects of IL-21 include induction of differentiation, maturation, and proliferation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Human IL-21 has also been shown to promote the anti-tumor activity of CD8+ T-cells and NK cells. During T-cell mediated immune response, IL21 may inhibit dendritic cells’ (DC) activation and maturation. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells, with the IL15, it may play a role in proliferation and maturation of natural killer (NK) cells. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78-81% and 58-67% homologous to the predicted human and murine IL-21 amino acid sequences, respectively. In one study, recombinant bovine IL-21 strongly induced NK cell proliferation using a human NK cell-line, NK0, and enhanced the lymphokine activated killer (LAK) activity of bovine peripheral blood mononuclear cells. In another by the same authors, recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MAT. Anti-IL-21 antibody is ideal for investigators involved in Cancer and Immunology research.
Sterilität Sterile filtered
Immunogen This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein.
Immunogen Type: RecombinantProtein
Isotyp IgG
Plasmids, Primers & others

Target Details IL21

Produktdetails anti-IL21 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung IL21 (IL21 Antibody Abstract)
Hintergrund Interleukin-21 (IL-21) is a secreted, type-I cytokine with immunoregulatory activity. Human IL-21 shares the common γ-chain with IL-2, IL-4, IL-7, IL-9, and IL-15 proteins but, in addition, binds to a unique IL-21Rα chain which triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Bovine and human IL-21 have pleiotropic functions and are mainly produced by activated T-cells in response to antigenic stimulation, but target a broad range of lymphoid and myeloid cells of the immune system (T cells, B cells, natural killer (NK) cells and dendritic cells). IL21 is therefore is able to regulate innate and acquired immune responses. The biological effects of IL-21 include induction of differentiation, maturation, and proliferation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Human IL-21 has also been shown to promote the anti-tumor activity of CD8+ T-cells and NK cells. During T-cell mediated immune response, IL21 may inhibit dendritic cells’ (DC) activation and maturation. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells; with the IL15, it may play a role in proliferation and maturation of natural killer (NK) cells. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78-81% and 58-67% homologous to the predicted human and murine IL-21 amino acid sequences, respectively. In one study, recombinant bovine IL-21 strongly induced NK cell proliferation using a human NK cell-line, NK0, and enhanced the lymphokine activated killer (LAK) activity of bovine peripheral blood mononuclear cells. In another by the same authors, recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MAT. Anti-IL-21 antibody is ideal for investigators involved in Cancer and Immunology research.
Synonyms: Interleukin-21, IL-21
Gen-ID 378475
UniProt Q76LU5
Pathways JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Anwendungsinformationen

Produktdetails anti-IL21 Antikörper Target Details IL21 Handhabung Bilder zurück nach oben
Applikations-hinweise IL-21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes, and in HL-60 and THP-1 cell lines. This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.1 kDa in size corresponding to bovine IL-21 protein by western blotting in the appropriate cell lysate or extract.
Kommentare

Gene Name: IL21

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-IL21 Antikörper Target Details IL21 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Rekonstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Haltbarkeit 12 months

Bilder

Produktdetails anti-IL21 Antikörper Target Details IL21 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Interleukin 21 (IL21) (AA 24-152) antibody (ABIN964709) Western blot using anti-bovine IL-21 antibody shows detection of recombinant bovine ...