anti-Hund HTR7 Antikörper für Immunohistochemistry

Recommended HTR7 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) Antikörper
  • 5-HT7
  • 5-HT-dro
  • 5-HT-dro1
  • 5-HT7Dro
  • 5-HT[7]Dro
  • 5-HT[[7Dro]]
  • 5-HT[[7]]
  • 5-HT[[7]]Dro
  • 5-HT[[dro1]]
  • 5-HTdro1
  • 5HT-7
  • 5HT-R1
  • 5HT-dro
  • 5HT-dro1
  • 5HT[[7Dro]]
  • 5htdror
  • CG12073
  • DRO1
  • Dm5HTdro1
  • Dmel\\CG12073
  • 5Ht7
  • 5HT7
  • htr7-A
  • 5-hydroxytryptamine receptor 7
  • 5-hydroxytryptamine (serotonin) receptor 7
  • 5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled
  • 5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled L homeolog
  • HTR7
  • 5-HT7
  • LOC752003
  • Htr7
  • htr7.L
Epitop
N-Term
51
17
10
8
4
3
3
3
2
2
2
1
1
1
1
Reaktivität
Hund, Human, Affe
117
21
19
17
9
8
7
3
3
3
3
2
2
2
2
1
1
1
Wirt
Kaninchen
103
16
Klonalität
Polyklonal
Konjugat
Dieser HTR7 Antikörper ist unkonjugiert
4
4
4
3
3
3
Applikation
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
53
51
43
23
13
4
2
2
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN1048278
$ 484.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
9.992777 ABIN213517 IHC IHC (p) Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0
9.992777 ABIN213559 ELISA IHC IHC (p) Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
6.992777 ABIN2469477 IHC ELISA Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
6.992777 ABIN2469437 IHC Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0

General

Antigen 5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) Antikörper
Epitop N-Term
(51), (17), (10), (8), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reaktivität Hund, Human, Affe
(117), (21), (19), (17), (9), (8), (7), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Wirt Kaninchen
(103), (16)
Klonalität
Polyklonal
Konjugat Dieser HTR7 Antikörper ist unkonjugiert
(4), (4), (4), (3), (3), (3)
Applikation Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(53), (51), (43), (23), (13), (4), (2), (2), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-HTR7 Antikörper

Target Details HTR7 Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human 5HT7 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Marmoset, Dog (100%) Mouse, Rat, Hamster, Panda (95%) Gorilla, Pig (90%) Horse (85%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 20 amino acid peptide from N-terminal extracellular domain of human 5HT7 Receptor. Percent identity with other species by BLAST analysis: Human, Marmoset, Dog (100%), Mouse, Rat, Hamster, Panda (95%), Gorilla, Pig (90%), Horse (85%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details HTR7

Produktdetails anti-HTR7 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung HTR7 / 5-HT7 Receptor (HTR7 Antibody Abstract)
Hintergrund Name/Gene ID: HTR7
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR7, 5-HT7, 5-HT7 receptor, 5-HT-7, 5-hydroxytryptamine 7 receptor, 5HT7, 5-HT-X, 5-hydroxytryptamine receptor 7, 5HT7 Receptor, HT7, Serotonin 5-HT7 receptor, Serotonin receptor 7
Gen-ID 3363
Pathways JAK-STAT Signalweg

Anwendungsinformationen

Produktdetails anti-HTR7 Antikörper Target Details HTR7 Handhabung Bilder zurück nach oben
Applikations-hinweise Approved: IHC, IHC-P (10 μg/mL)
Kommentare

Target Species of Antibody: Human

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-HTR7 Antikörper Target Details HTR7 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-HTR7 Antikörper Target Details HTR7 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) (N-Term) antibody (ABIN1048278) Anti-5HT7 Receptor antibody ABIN1048278 IHC staining of human brain, cerebellum. Imm...
Immunohistochemistry (IHC) image for anti-5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7) (N-Term) antibody (ABIN1048278) Anti-HTR7 / 5-HT7 Receptor antibody IHC of human Brain, Glioblastoma. Immunohistochem...