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|Antigen||Angiotensin II (Ang II) ELISA Kits|
Kits mit alternativen Reaktivitäten:
|Detektionsbereich||24.69 pg/mL - 2000 pg/mL|
|Untere Nachweisgrenze||24.69 pg/mL|
|9 Publikationen vorhanden|
|Hersteller||Anmelden zum Anzeigen|
Produktdetails Angiotensin II ELISA KitAntigendetails Anwendungsinformationen Handhabung Referenzen für Angiotensin II Kit (ABIN414350) Bilder
|Verwendungszweck||The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AngII in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids|
|Proben||Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids|
This assay has high sensitivity and excellent specificity for detection of Angiotensin II (AngII).
|Kreuzreaktivität (Details)||No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.|
AntigendetailsProduktdetails Angiotensin II ELISA Kit Anwendungsinformationen Handhabung Referenzen für Angiotensin II Kit (ABIN414350) Bilder zurück nach oben
|Andere Bezeichnung||AngII (Ang II ELISA Kit Abstract)|
|Pathways||JAK-STAT Signalweg, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity|
AnwendungsinformationenProduktdetails Angiotensin II ELISA Kit Antigendetails Handhabung Referenzen für Angiotensin II Kit (ABIN414350) Bilder zurück nach oben
Information on standard material:
|Protokoll||This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Angiotensin II (AngII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Angiotensin II (AngII) and unlabeled Angiotensin II (AngII) (Standards or samples) with the pre-coated antibody specific to Angiotensin II (AngII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin II (AngII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Angiotensin II (AngII) in the sample.|
|Aufbereitung der Reagenzien||
Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Tissue Homogenates: The preparation of tissue homogenates will vary depending upon tissue type. For this assay, rinse tissues in ice-cold PBS (0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weigh before homogenization. Mince the tissues to small pieces and homogenize them in 5-10 mL of PBS with a glass homogenizer on ice (Micro Tissue Grinders work, too). Sonicate the resulting suspension with an ultrasonic cell disrupter or subject it to two freeze-thaw cycles to further break the cell membranes. Centrifugate the homogenates for 5 minutes at 5000 × g. Remove the supernate and assay immediately or aliquot and store at -20°C
Cell Lysate: Cells must be lysed before assaying according to the following directions. Adherent cells should be detached with trypsin and then collected by centrifugation (suspension cells can be collected by centrifugation directly). Wash cells three times in cold PBS. Resuspend cells in PBS (1×) and subject them to ultrasonication for 4 times (or Freeze cells at -20 °C. Thaw cells with gentle mixing. Repeat the freeze/thaw cycle for 3 times.) Centrifuge at 1500 × g for 10 minutes at 2 - 8°C to remove cellular debris.
Cell Culture Supernatant: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Aufbereitung der Proben||
This assay employs the competitive inhibition enzyme immunoassay technique, so there is an inverse correlation between AngII concentration in the sample and the assay signal intensity. Average the duplicate readings for each standard, control, and samples. Create a standard curve on log-log or semi-log graph paper, with the log of AngII concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the log of concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. The O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects). Typical standard curve below is provided for reference only.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II (AngII) were tested 20 times on one plate, respectively.
|Beschränkungen||Nur für Forschungszwecke einsetzbar|
HandhabungProduktdetails Angiotensin II ELISA Kit Antigendetails Anwendungsinformationen Referenzen für Angiotensin II Kit (ABIN414350) Bilder zurück nach oben
|Vorsichtsmaßnahmen||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
|Informationen zur Lagerung||
Referenzen für Angiotensin II Kit (ABIN414350)Produktdetails Angiotensin II ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
|Produkt verwendet in:||
Zheng, Wang, Zou, Jing, Yang, Li, Wang: "Ginsenoside Rb1 improves cardiac function and remodeling in heart failure." in: Experimental animals, Vol. 66, Issue 3, pp. 217-228, 2017
Jeong, Hong, Cho, Lee, Kwon, Joo, Park, Yu, Lim: "Effects of Rubus occidentalis extract on blood pressure in patients with prehypertension: Randomized, double-blinded, placebo-controlled clinical trial." in: Nutrition (Burbank, Los Angeles County, Calif.), Vol. 32, Issue 4, pp. 461-7, 2016
Clancy, Koblar, Golledge: "Involvement of Angiotensin II Type 1 and 2 Receptors in Gelatinase Regulation in Human Carotid Atheroma in vitro." in: Journal of atherosclerosis and thrombosis, Vol. 23, Issue 7, pp. 773-91, 2016
Qu, Hui, Wang, Tang, Zhong, Liu, Li, Feng, He et al.: "Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of ..." in: PLoS ONE, Vol. 11, Issue 7, pp. e0157456, 2016
Chang, Lee, Chen, Lin, Hsu, Chan, Chang, Chang, Pan, Sawamura, Chang, Su, Chen: "Increased LDL electronegativity in chronic kidney disease disrupts calcium homeostasis resulting in cardiac dysfunction." in: Journal of molecular and cellular cardiology, Vol. 84, pp. 36-44, 2015
Huang, Guo, Sun, Chen, Yin: "Angiotensin II promotes the progression of human gastric cancer." in: Molecular medicine reports, Vol. 9, Issue 3, pp. 1056-60, 2014
Wang, Sjöberg, Tang, Oörni, Wu, Liu, Secco, Tia, Sukhova, Fernandes, Lesner, Kovanen, Libby, Cheng, Shi: "Cathepsin G activity lowers plasma LDL and reduces atherosclerosis." in: Biochimica et biophysica acta, Vol. 1842, Issue 11, pp. 2174-83, 2014
Zhang, Long, Sun, Wang, Li, Wu, Guo, Li, Niu, Li, Liu, Mei et al.: "Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by ..." in: European journal of pharmacology, Vol. 651, Issue 1-3, pp. 187-96, 2010
Wang, Liu, Peng, Tang, Tang, Chen, Lou, Zhang: "Mesangial cells stimulated by immunoglobin A1 from IgA nephropathy upregulates transforming growth factor-beta1 synthesis in podocytes via renin-angiotensin system activation." in: Archives of medical research, Vol. 41, Issue 4, pp. 255-60, 2010
BilderProduktdetails Angiotensin II ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für Angiotensin II Kit (ABIN414350) zurück nach oben
|Bilder des Herstellers||