Rattete IgG2a Isotyp-Kontrolle
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- Target Alle IgG2a Produkte
- IgG2a
- Reaktivität
- Ratte
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Wirt
- Ratte
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Klonalität
- Monoklonal
- Applikation
- Flow Cytometry (FACS), ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Aufreinigung
- RAT IgG2a Kappa (κ) isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1a, IgG1b, IgG3 , IgM , and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
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Klon
- RG2aK
- Isotyp
- IgG2a
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Mouse IgG2a isotype control (APC)
FACS Monoclonal MOPC-173 IgG2a kappa APC
Mouse IgG2a isotype control (PerCP)FACS Monoclonal MOPC-173 IgG2a kappa PerCP
Mouse IgG2a isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Mouse IgG2a isotype controlFACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Rat IgG2a isotype control (FITC)FACS Monoclonal RTG2A1-1 IgG2a FITC
Mouse IgG2a isotype control (Biotin)FACS, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa Biotin
Mouse IgG2a isotype control (FITC)FACS Monoclonal MOPC-173 IgG2a kappa FITC
Mouse IgG2a isotype control (PE)FACS Monoclonal MOPC-173 IgG2a kappa PE
Mouse IgG2a isotype control (FITC)FACS, IsoC, IF IgG2a FITC
Mouse IgG2a isotype control (FITC)FACS, ELISA Monoclonal HOPC-1 IgG2a FITC
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- Applikationshinweise
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: RAT IgG2a Kappa (κ) isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
- Kommentare
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RAT IgG2a Kappa (κ) isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain α - IgA, γ - IgG 1, 2a, 2b, 2c and μ - IgM, light chain κ and λ.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Stabilizer: None
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Haltbarkeit
- 12 months
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- Target
- IgG2a
- Abstract
- IgG2a Produkte
- Synonyme
- gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
- Substanzklasse
- Antibody
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