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Maus IgG3 isotype control (APC)

FACS, ELISA, FM, FLISA Monoclonal IgG3 APC
Produktnummer ABIN5608804
  • Target Alle IgG3 Produkte
    IgG3
    Reaktivität
    Maus
    Wirt
    • 60
    • 8
    • 5
    • 1
    • 1
    Maus
    Klonalität
    • 41
    • 2
    • 1
    Monoklonal
    Konjugat
    APC
    Applikation
    Flow Cytometry (FACS), ELISA, Fluorescence Microscopy (FM), FLISA
    Aufreinigung
    Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Light and heavy chain composition has been confirmed.
    Isotyp
    IgG3
    Labeling Ratio
    1-2
  • Applikationshinweise

    Flow Cytometry Dilution: 1:1000-1:5000

    Application Note: Mouse IgG3 kappa isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

    FLISA Dilution: User Optimized

    ELISA Dilution: 1:2000-1:20,000

    IF Microscopy Dilution: User Optimized

    Kommentare

    Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain α - IgA, γ - IgG 1, 2a, 2b, 3 and μ - IgM, light chain κ and λ.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Stabilizer: None

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
    Haltbarkeit
    12 months
  • Target
    IgG3
    Abstract
    IgG3 Produkte
    Synonyme
    IgG3 Isotype Control, Immunoglobulin heavy constant gamma 3 Isotype Control, immunoglobulin heavy constant gamma 3 (G3m marker) Isotype Control, Ighg3 Isotype Control, IGHG3 Isotype Control
    Substanzklasse
    Antibody
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