anti-Human MTOR Antikörper für Western Blotting

Recommended MTOR Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antikörper
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin kinase
  • mechanistic target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitop
pSer2448
67
31
29
22
21
18
16
15
15
11
7
7
6
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Fisch, Human, Maus, Ratte (Rattus)
407
221
211
16
8
6
6
6
6
5
4
3
1
1
1
1
Wirt
Kaninchen
355
49
3
Klonalität
Polyklonal
Konjugat
Dieser MTOR Antikörper ist unkonjugiert
10
10
9
5
4
4
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
294
155
127
69
59
52
33
19
16
8
8
7
3
3
2
2
2
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN6255124
$ 390.77
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.1357207 ABIN6263424 ELISA ICC IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 7
3.1357207 ABIN2855088 ChIP ICC IF IP IHC (p) WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 0
3.1357207 ABIN153493 ELISA EM IHC IHC (p) WB Rabbit IgG pSer2448 Anmelden zum Anzeigen Polyclonal 5
3.1357207 ABIN1532744 ELISA WB Rabbit IgG AA 2412-2461 Anmelden zum Anzeigen Polyclonal 3
3.1357207 ABIN1531715 ELISA WB Rabbit IgG AA 2412-2461, pThr2446 Anmelden zum Anzeigen Polyclonal 3
3.1357207 ABIN4880882 ELISA IHC WB Mouse IgG2a AA 2311-2529 Anmelden zum Anzeigen 8H5A5 0
3.1357207 ABIN151707 ICC IF IP IHC PLA WB Rabbit AA 200-250 Anmelden zum Anzeigen Polyclonal 4
3.1357207 ABIN2705013 IC IF WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
3.1357207 ABIN6270019 ELISA IHC WB Rabbit IgG pSer2454 Anmelden zum Anzeigen Polyclonal 0
3.1357207 ABIN1531910 IHC ELISA WB Rabbit IgG AA 2415-2464, pSer2448 Anmelden zum Anzeigen Polyclonal 1
3.1357207 ABIN390217 IF IHC (p) WB Rabbit Ig Fraction AA 2459-2488, Ser2481 Anmelden zum Anzeigen Polyclonal 3
3.1357207 ABIN1532464 IHC ELISA WB Rabbit IgG AA 2447-2496 Anmelden zum Anzeigen Polyclonal 0
3.1357207 ABIN1532942 IHC ELISA WB Rabbit IgG AA 2415-2464 Anmelden zum Anzeigen Polyclonal 0
3.1357207 ABIN6256522 ELISA WB Rabbit IgG pThr2446 Anmelden zum Anzeigen Polyclonal 2
3.1357207 ABIN4880883 ELISA WB Mouse IgG2a AA 2311-2529 Anmelden zum Anzeigen 5E4D8 1
3.1357207 ABIN6256211 ELISA ICC IF IHC WB Rabbit IgG pSer2481 Anmelden zum Anzeigen Polyclonal 1
3.1357207 ABIN2855089 WB Rabbit IgG Internal Region Anmelden zum Anzeigen Polyclonal 0
3.1357207 ABIN129663 ELISA WB Rabbit IgG AA 2440-2457 Anmelden zum Anzeigen Polyclonal 0
3.1357207 ABIN1868039 ICC IHC WB Rabbit IgG AA 2226-2488 Anmelden zum Anzeigen Polyclonal 0
3.1357207 ABIN3185709 ELISA IF IHC WB Rabbit IgG Ser396 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antikörper
Epitop pSer2448
(67), (31), (29), (22), (21), (18), (16), (15), (15), (11), (7), (7), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Fisch, Human, Maus, Ratte (Rattus)
(407), (221), (211), (16), (8), (6), (6), (6), (6), (5), (4), (3), (1), (1), (1), (1)
Wirt Kaninchen
(355), (49), (3)
Klonalität
Polyklonal
Konjugat Dieser MTOR Antikörper ist unkonjugiert
(10), (10), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(294), (155), (127), (69), (59), (52), (33), (19), (16), (8), (8), (7), (3), (3), (2), (2), (2), (1), (1)
Pubmed 12 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MTOR Antikörper

Target Details MTOR Anwendungsinformationen Handhabung Referenzen für anti-MTOR Antikörper (ABIN6255124) Bilder
Spezifität Phospho-mTOR (Ser2448) Antibody detects endogenous levels of mTOR only when phosphorylated at Serine 2448
Kreuzreaktivität Fisch, Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human mTOR around the phosphorylation site of Serine 2448
Isotyp IgG
Plasmids, Primers & others

Target Details MTOR

Produktdetails anti-MTOR Antikörper Anwendungsinformationen Handhabung Referenzen für anti-MTOR Antikörper (ABIN6255124) Bilder zurück nach oben
Antigen
Andere Bezeichnung mTOR (FRAP1 Antibody Abstract)
Hintergrund

Description: Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422' (PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:12231510, PubMed:12718876, PubMed:14651849, PubMed:15268862, PubMed:15467718, PubMed:15545625, PubMed:15718470, PubMed:18497260, PubMed:18762023, PubMed:18925875, PubMed:20516213, PubMed:20537536, PubMed:21659604, PubMed:23429703, PubMed:23429704, PubMed:25799227, PubMed:26018084). Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms (By similarity).

Gene: MTOR

Molekulargewicht 289kDa
Gen-ID 2475
UniProt P42345
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events

Anwendungsinformationen

Produktdetails anti-MTOR Antikörper Target Details MTOR Handhabung Referenzen für anti-MTOR Antikörper (ABIN6255124) Bilder zurück nach oben
Applikations-hinweise WB 1:500-1:2000 IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Referenzen für anti-MTOR Antikörper (ABIN6255124) Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months

Referenzen für anti-MTOR Antikörper (ABIN6255124)

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Song, Zhang, Sun, Li, Chen, Xiao, Xing: "AMPK activation-dependent autophagy compromises oleanolic acid-induced cytotoxicity in human bladder cancer cells." in: Oncotarget, Vol. 8, Issue 40, pp. 67942-67954, 2018

Xue, Ding, Ji, Yan, Huang, Guo, Yang, Sun: "PD149163 induces hypothermia to protect against brain injury in acute cerebral ischemic rats." in: Journal of pharmacological sciences, Vol. 135, Issue 3, pp. 105-113, 2018

Huang, Chen, Zhang, Li, Chen, Yang, Liang, Zhu, Xu: "Neuroprotective Effect of Curcumin Against Cerebral Ischemia-Reperfusion Via Mediating Autophagy and Inflammation." in: Journal of molecular neuroscience : MN, Vol. 64, Issue 1, pp. 129-139, 2018

Li, Li, Zeng, Yang, Liu, Zhang, Song, Yao, Ma, Li, Wang, Wei: "Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis." in: Journal of virology, Vol. 92, Issue 8, 2018

Wu, Zhu, Yin, Kang, Cao, Tian, Zhang, Lu, Liu: "Traditional Chinese Medicine CFF-1 induced cell growth inhibition, autophagy, and apoptosis via inhibiting EGFR-related pathways in prostate cancer." in: Cancer medicine, Vol. 7, Issue 4, pp. 1546-1559, 2018

Zheng, Feng, Jiang, Wu, Tang, Kuang, Zeng, Zhou, Liu: "Selenium deficiency impaired immune function of the immune organs in young grass carp (Ctenopharyngodon idella)." in: Fish & shellfish immunology, Vol. 77, pp. 53-70, 2018

Wu, Zhu, Zhang, Yin, Kang, Cao, Tian, Lu, Liu: "EGFR‑associated pathways involved in traditional Chinese medicine (TCM)‑1‑induced cell growth inhibition, autophagy and apoptosis in prostate cancer." in: Molecular medicine reports, Vol. 17, Issue 6, pp. 7875-7885, 2018

Jin, Hu, Ye, Wu, He: "Rapamycin Reduces Podocyte Apoptosis and is Involved in Autophagy and mTOR/ P70S6K/4EBP1 Signaling." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 48, Issue 2, pp. 765-772, 2018

Zhao, Deng, Xu, Zhang, Mi, Meng, Gou, Xu: "PirB Overexpression Exacerbates Neuronal Apoptosis by Inhibiting TrkB and mTOR Phosphorylation After Oxygen and Glucose Deprivation Injury." in: Cellular and molecular neurobiology, Vol. 37, Issue 4, pp. 707-715, 2017

He, Cao, Guo, Li, Shang, Liu, Xie, Xu, Liu: "Quercetin induces autophagy via FOXO1-dependent pathways and autophagy suppression enhances quercetin-induced apoptosis in PASMCs in hypoxia." in: Free radical biology & medicine, Vol. 103, pp. 165-176, 2017

Wu, Huang, Jiao, Ding, Zhang, Chen, Wang, Li, Huo: "Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells." in: Oncotarget, Vol. 8, Issue 51, pp. 88630-88644, 2017

Liu, Luo, Mu, Liu, Geng, Liu, Yi: "Fluoxetine regulates mTOR signalling in a region-dependent manner in depression-like mice." in: Scientific reports, Vol. 5, pp. 16024, 2016

Bilder

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Handhabung Referenzen für anti-MTOR Antikörper (ABIN6255124) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN6255124) ABIN6267517 at 1/50 staining human lung cancer tissue sections by IHC-P. The tissue w...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN6255124) ABIN6267517 at 1/100 staining human prostate cancer tissues sections by IHC-P. The ti...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN6255124) ABIN6267517 at 1/50 staining human lung cancer tissue sections by IHC-P. The tissue w...
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN6255124) Western blot analysis of mTOR phosphorylation expression in EGF treated HeLa whole ce...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN6255124) Phospho-mTOR (Ser2448) Antibody for IHC in human prostate tissue
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN6255124) Western blot analysis of mTOR phosphorylation expression in mouse liver tissue lysate...