anti-Maus Sonic Hedgehog Antikörper für Western Blotting

Recommended Sonic Hedgehog Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Sonic Hedgehog (SHH) Antikörper
  • shh
  • SHH
  • 9530036O11Rik
  • Dsh
  • Hhg1
  • Hx
  • Hxl3
  • M100081
  • HHG1
  • HLP3
  • HPE3
  • MCOPCB5
  • SMMCI
  • TPT
  • TPTPS
  • Xhh
  • hedgehog
  • xshh
  • fc83d08
  • syu
  • vhh-1
  • vhh1
  • wu:fc83d08
  • sonic hedgehog
  • sonic hedgehog protein A
  • sonic hedgehog L homeolog
  • sonic hedgehog a
  • shh
  • SHH
  • Shh
  • shh.L
  • shha
Epitop
N-Term
16
15
13
12
5
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Hund, Ziege, Meerschweinchen, Pferd, Human, Maus, Kaninchen, Ratte (Rattus), Zebrafisch (Danio rerio)
108
79
58
12
8
7
5
2
2
1
1
1
1
1
1
1
1
Wirt
Kaninchen
106
42
10
1
Klonalität
Polyklonal
Konjugat
Dieser Sonic Hedgehog Antikörper ist unkonjugiert
8
4
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (IHC), Western Blotting (WB)
129
56
50
28
20
14
13
13
11
10
5
3
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN2781775
$ 289.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.0625389 ABIN731108 IF (p) IHC (p) WB Rabbit IgG AA 20-70 Anmelden zum Anzeigen Polyclonal 8
3.0625389 ABIN4881046 ELISA FACS IHC WB Mouse IgG1 AA 26-161 Anmelden zum Anzeigen 5H4 0
3.0625389 ABIN4355158 ELISA FACS IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 5H4 2
3.0625389 ABIN969567 FACS IHC ELISA WB Mouse IgG1 AA 26-161 Anmelden zum Anzeigen 5H4 1
3.0625389 ABIN2862639 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN1846141 FACS IHC ELISA WB Mouse IgG1 AA 26-161 Anmelden zum Anzeigen 0
3.0625389 ABIN1109100 IF IHC (p) WB Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN4355160 ICC IF IHC IHC (p) WB Rabbit AA 1-75, N-Term Anmelden zum Anzeigen Polyclonal 1
3.0625389 ABIN1175085 ICC IHC WB Rabbit IgG AA 28-193 Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN1874775 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN2462795 IHC ELISA WB Rabbit Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN5970002 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN5968864 IHC (p) WB Rabbit Ig Fraction C-Term Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN5968865 IHC (p) WB Rabbit Ig Fraction C-Term Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN635696 IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN3029025 FACS ELISA WB Rabbit Ig Fraction AA 395-450 Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN3032696 ELISA WB Rabbit Ig Fraction N-Term Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN3032697 ELISA WB Rabbit Ig Fraction C-Term Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN3032695 IHC ELISA WB Rabbit Ig Fraction C-Term Anmelden zum Anzeigen Polyclonal 0
3.0625389 ABIN2932539 IF/ICC IHC IP WB Rabbit AA 28-193 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Sonic Hedgehog (SHH) Antikörper
Epitop N-Term
(16), (15), (13), (12), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Rind (Kuh), Hund, Ziege, Meerschweinchen, Pferd, Human, Maus, Kaninchen, Ratte (Rattus), Zebrafisch (Danio rerio)
(108), (79), (58), (12), (8), (7), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(106), (42), (10), (1)
Klonalität
Polyklonal
Konjugat Dieser Sonic Hedgehog Antikörper ist unkonjugiert
(8), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (IHC), Western Blotting (WB)
(129), (56), (50), (28), (20), (14), (13), (13), (11), (10), (5), (3), (1), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Sonic Hedgehog Antikörper

Target Details Sonic Hedgehog Anwendungsinformationen Handhabung Referenzen für anti-Sonic Hedgehog Antikörper (ABIN2781775) Bilder
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN983711.
Sequenz RCLLLVLVSS LLVCSGLACG PGRGFGKRRH PKKLTPLAYK QFIPNVAEKT
Homologie Cow: 93%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
Produktmerkmale This is a rabbit polyclonal antibody against SHH. It was validated on Western Blot and immunohistochemistry.
Reinigung Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human SHH
Plasmids, Primers & others

Target Details Sonic Hedgehog

Produktdetails anti-Sonic Hedgehog Antikörper Anwendungsinformationen Handhabung Referenzen für anti-Sonic Hedgehog Antikörper (ABIN2781775) Bilder zurück nach oben
Antigen
Andere Bezeichnung SHH (SHH Antibody Abstract)
Hintergrund SHH is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in its gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.This gene, which is expressed only during embryogenesis, encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
Alias Symbols: HHG1, HLP3, HPE3, SMMCI, TPT, TPTPS, MCOPCB5
Protein Interaction Partner: UBC, SEL1L, DERL2, DERL1, SYVN1, HHIP, PTCH2, PTCH1, SHH, ADCYAP1, GAS1,
Protein Size: 462
Molekulargewicht 28 kDa
Gen-ID 6469
NCBI Accession NM_000193, NP_000184
UniProt Q15465
Pathways Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development

Anwendungsinformationen

Produktdetails anti-Sonic Hedgehog Antikörper Target Details Sonic Hedgehog Handhabung Referenzen für anti-Sonic Hedgehog Antikörper (ABIN2781775) Bilder zurück nach oben
Applikations-hinweise Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

Antigen size: 462 AA

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Sonic Hedgehog Antikörper Target Details Sonic Hedgehog Anwendungsinformationen Referenzen für anti-Sonic Hedgehog Antikörper (ABIN2781775) Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-Sonic Hedgehog Antikörper (ABIN2781775)

Produktdetails anti-Sonic Hedgehog Antikörper Target Details Sonic Hedgehog Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Coon, Roberts, Loscertales, Kradin: "Differential epithelial expression of SHH and FOXF1 in usual and nonspecific interstitial pneumonia." in: Experimental and molecular pathology, Vol. 80, Issue 2, pp. 119-23, 2006

Bilder

Produktdetails anti-Sonic Hedgehog Antikörper Target Details Sonic Hedgehog Anwendungsinformationen Handhabung Referenzen für anti-Sonic Hedgehog Antikörper (ABIN2781775) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Sonic Hedgehog (SHH) (N-Term) antibody (ABIN2781775) WB Suggested Anti-SHH Antibody Titration: 0.2-1 ug/ml Positive Control: HepG2 cell...
Immunohistochemistry (IHC) image for anti-Sonic Hedgehog (SHH) (N-Term) antibody (ABIN2781775) Sample Type: Chicken EmbryosDilution: 1:1000
Immunohistochemistry (IHC) image for anti-Sonic Hedgehog (SHH) (N-Term) antibody (ABIN2781775) Chicken embryos Primary antibody: 1:1000 (ARP44235_p050) anti -shh Secondary Antibod...
Western Blotting (WB) image for anti-Sonic Hedgehog (SHH) (N-Term) antibody (ABIN2781775) Host: Rabbit Target Name: SHH Sample Tissue: Human Stomach Tumor Antibody Dilut...
Western Blotting (WB) image for anti-Sonic Hedgehog (SHH) (N-Term) antibody (ABIN2781775) Host: Mouse Target Name: SHH Sample Tissue: Mouse Brain Antibody Dilution: 1ug/ml
Immunohistochemistry (IHC) image for anti-Sonic Hedgehog (SHH) (N-Term) antibody (ABIN2781775) Sample Type : Human glioma cells Primary Antibody Dilution : 1:200 Secondary Anti...
Immunohistochemistry (IHC) image for anti-Sonic Hedgehog (SHH) (N-Term) antibody (ABIN2781775) Rabbit Anti-SHH Antibody Formalin Fixed Paraffin Embedded Tissue: Human Urinary Bla...