anti-Human Ubiquitin B Antikörper für Immunofluorescence

Recommended Ubiquitin B Antibody

Antigen
Ubiquitin B (UBB) Antikörper
  • AL033289
  • Rps27a
  • Uba52
  • Ubb2
  • Ubc
  • UBC
  • Ubiquitin
  • UBB
  • Ubi-p63E
  • im:6892314
  • si:ch211-202a12.3
  • ubc
  • zUBC
  • zgc:172187
  • ubiquitin B
  • UBB
  • Ubb
  • ubb
Epitop
AA 1-119
5
2
2
2
2
1
1
1
1
1
1
1
Reaktivität
Human
60
39
33
13
3
3
3
3
3
3
2
2
2
2
2
1
1
1
Wirt
Maus
49
23
Klonalität
Monoklonal
Konjugat
Dieser Ubiquitin B Antikörper ist unkonjugiert
4
2
2
2
2
2
2
2
2
2
2
Applikation
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
55
29
27
15
14
12
6
5
3
2
1
1
Optionen
Produktnummer ABIN6940844
$ 522.06
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Clonality References Details
13.477342 ABIN6938717 FACS IF IHC StM WB Mouse IgG2c, kappa AA 1-119 UBB-1748 0
13.477342 ABIN6135950 IF IHC WB Rabbit 0
4.8147545 ABIN5947900 ICC IF IHC IHC (p) WB Rabbit IgG AA 50-150 JM09-67 0
1 ABIN2629705 ICC IF WB Rabbit IgG AA 1-179 Polyclonal 0
1 ABIN6675617 IF IHC WB Rabbit 0
-13.1852455 ABIN4363980 ELISA ICC IF WB Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628289 ELISA ICC IF WB Alexa Fluor 405 Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628290 ELISA ICC IF WB Alexa Fluor 488 Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628291 ELISA ICC IF WB Alexa Fluor 647 Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628292 ELISA ICC IF WB Alexa Fluor 700 Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628293 ELISA ICC IF WB Biotin Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628294 ELISA ICC IF WB DyLight 350 Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628296 ELISA ICC IF WB DyLight 488 Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628297 ELISA ICC IF WB DyLight 550 Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628295 ELISA ICC IF WB DyLight 405 Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628298 ELISA ICC IF WB DyLight 650 Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628299 ELISA ICC IF WB DyLight 680 Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628300 ELISA ICC IF WB DyLight 755 Mouse IgG2a kappa 6C11-B3 0
-13.1852455 ABIN5628301 ELISA ICC IF FITC Mouse IgG2a kappa 6C11-B3 0

General

Antigen Ubiquitin B (UBB) Antikörper
Epitop AA 1-119
(5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(60), (39), (33), (13), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Wirt Maus
(49), (23)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Ubiquitin B Antikörper ist unkonjugiert
(4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
(55), (29), (27), (15), (14), (12), (6), (5), (3), (2), (1), (1)
Hersteller

Produktdetails anti-Ubiquitin B Antikörper

Target Details Ubiquitin B Anwendungsinformationen Handhabung Bilder
Reinigung Purified by Protein A/G
Immunogen Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)
Klon UBB-1748
Isotyp IgG2c, kappa
Plasmids, Primers & others

Target Details Ubiquitin B

Produktdetails anti-Ubiquitin B Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung UBB (UBB Antibody Abstract)
Hintergrund Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.
Molekulargewicht 9kDa
Gen-ID 7314
UniProt P62979, P62987, P62988
Pathways Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, EGFR Downregulation

Anwendungsinformationen

Produktdetails anti-Ubiquitin B Antikörper Target Details Ubiquitin B Handhabung Bilder zurück nach oben
Applikations-hinweise

Positive Control: MCF-7, HeLa or Jurkat cells. Alzheimer's Brain.

Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Ubiquitin B Antikörper Target Details Ubiquitin B Anwendungsinformationen Bilder zurück nach oben
Konzentration 200 μg/mL
Buffer 10 mM PBS with 0.05 % BSA & 0.05 % azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-80 °C
Informationen zur Lagerung Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Haltbarkeit 24 months