anti-Human Nuclear Receptor Subfamily 4, Group A, Member 3 Antikörper für Immunohistochemistry

Recommended Nuclear Receptor Subfamily 4, Group A, Member 3 Antibody

Antigen
Nuclear Receptor Subfamily 4, Group A, Member 3 (NR4A3) Antikörper
  • CHN
  • CSMF
  • MINOR
  • NOR1
  • TEC
  • AI573420
  • NOR-1
  • Nor1
  • NOR-2
  • NR4A3
  • Nr4a3
  • nuclear receptor subfamily 4 group A member 3
  • nuclear receptor subfamily 4, group A, member 3
  • NR4A3
  • Nr4a3
  • nr4a3
Epitop
Internal Region
22
17
16
16
12
10
9
9
9
8
8
7
5
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Hund, Human, Schwein, Kaninchen
186
84
63
7
6
5
5
5
4
4
4
3
2
Wirt
Kaninchen
171
36
Klonalität
Polyklonal
Konjugat
Unkonjugiert
11
10
10
8
8
8
3
3
3
3
3
3
2
2
2
2
2
Applikation
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
156
86
34
30
26
8
3
3
3
2
Optionen

Bilder

Produktnummer ABIN1049141
$ 484.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Clonality References Details
13.082039 ABIN3003456 IF/ICC IHC WB Rabbit IgG Polyclonal 0
10.082039 ABIN1049140 IHC IHC (p) Rabbit Modulating Domain Polyclonal 0
10.082039 ABIN1049142 IHC IHC (p) Rabbit C-Term Polyclonal 0
10 ABIN2451820 IF IHC IHC (p) WB Rabbit AA 175-205 Polyclonal 0
7.9152493 ABIN4340623 IHC IHC (p) WB Mouse IgG1 5C2 0
7.0820394 ABIN6755438 IHC IHC (p) WB Rabbit IgG AA 351-400 Polyclonal 0
5.5 ABIN2727595 IHC WB Mouse IgG1 AA 332-626 OTI2E1 1
1 ABIN2626154 IHC IHC (p) WB Mouse IgG1 AA 332-626 2A6 0
1 ABIN2626153 IHC IHC (p) WB Mouse IgG1 AA 332-626 2B11 0
1 ABIN2626155 IHC IHC (p) WB Mouse IgG1 AA 332-626 2E1 0
1 ABIN2629009 IHC IHC (p) WB Rabbit IgG AA 612-631 Polyclonal 0
1 ABIN1941185 IHC ELISA WB PE Rabbit IgG AA 175-205 Polyclonal 0
1 ABIN1941182 IHC ELISA WB APC Rabbit IgG AA 175-205 Polyclonal 0
1 ABIN1941184 IHC ELISA WB FITC Rabbit IgG AA 175-205 Polyclonal 0
1 ABIN1941181 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 175-205 Polyclonal 0
1 ABIN2573760 ELISA IHC WB Rabbit IgG AA 175-205 Polyclonal 0
1 ABIN2373149 IHC WB Rabbit IgG C-Term Polyclonal 0
1 ABIN1941186 IHC ELISA WB HRP Rabbit IgG AA 175-205 Polyclonal 0
1 ABIN1941183 IHC ELISA WB Biotin Rabbit IgG AA 175-205 Polyclonal 0
1 ABIN2373155 IHC ELISA WB Rabbit IgG Center, Internal Region Polyclonal 0

General

Antigen Nuclear Receptor Subfamily 4, Group A, Member 3 (NR4A3) Antikörper
Epitop Internal Region
(22), (17), (16), (16), (12), (10), (9), (9), (9), (8), (8), (7), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Rind (Kuh), Hund, Human, Schwein, Kaninchen
(186), (84), (63), (7), (6), (5), (5), (5), (4), (4), (4), (3), (2)
Wirt Kaninchen
(171), (36)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(11), (10), (10), (8), (8), (8), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2)
Applikation Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(156), (86), (34), (30), (26), (8), (3), (3), (3), (2)
Hersteller

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human NR4A3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bovine, Dog, Elephant, Panda, Rabbit, Pig, Platypus (100%) Marmoset, Mouse, Rat, Bat, Hamster, Opossum, Turkey, Chicken (94%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 17 amino acid peptide from internal region of human NR4A3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bovine, Dog, Elephant, Panda, Rabbit, Pig, Platypus (100%), Marmoset, Mouse, Rat, Bat, Hamster, Opossum, Turkey, Chicken (94%).

Type of Immunogen: Synthetic peptide

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Nor-1 / NR4A3 (NR4A3 Antibody Abstract)
Hintergrund Name/Gene ID: NR4A3
Subfamily: NR4 Nerve growth factor-like
Family: NHR

Synonyms: NR4A3, CHN, MINOR, NOR1, Neuron-derived orphan receptor, Nuclear hormone receptor NOR-1, Nor-1, CSMF, Orphan receptor nor1, TEC receptor
Gen-ID 8013
Pathways Fc-epsilon Rezeptor Signalübertragung, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Bilder zurück nach oben
Applikations-hinweise Approved: IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
Kommentare

Target Species of Antibody: Human

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 4, Group A, Member 3 (NR4A3) (Internal Region) antibody (ABIN1049141) Anti-NR4A3 antibody ABIN1049141 IHC staining of human brain, cortex. Immunohistochem...
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 4, Group A, Member 3 (NR4A3) (Internal Region) antibody (ABIN1049141) Anti-NR4A3 antibody ABIN1049141 IHC staining of human skeletal muscle. Immunohistoch...
Immunohistochemistry (IHC) image for anti-Nuclear Receptor Subfamily 4, Group A, Member 3 (NR4A3) (Internal Region) antibody (ABIN1049141) Anti-Nor-1 / NR4A3 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistoch...