anti-Human Nuclear Receptor Subfamily 4, Group A, Member 3 Antikörper für Immunohistochemistry
Recommended Nuclear Receptor Subfamily 4, Group A, Member 3 Antibody
- Antigen
-
Nuclear Receptor Subfamily 4, Group A, Member 3 (NR4A3) Antikörper
Synonyme für dieses Antigen anzeigen- CHN
- CSMF
- MINOR
- NOR1
- TEC
- AI573420
- NOR-1
- Nor1
- NOR-2
- NR4A3
- Nr4a3
- nuclear receptor subfamily 4 group A member 3
- nuclear receptor subfamily 4, group A, member 3
- NR4A3
- Nr4a3
- nr4a3
- Epitop
-
Internal Region
Alternativen - Reaktivität
-
Rind (Kuh), Hund, Human, Schwein, Kaninchen
Alternativen - Wirt
-
Kaninchen
Alternativen - Klonalität
- Konjugat
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Unkonjugiert
Alternativen - Applikation
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Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Alternativen - Optionen
Produktnummer ABIN1049141
$ 484.00
Zzgl. Versandkosten $45.00
Zzgl. Versandkosten $45.00
Relevance Score | ABIN | Application | Konjugat | Host | Isotype | Epitope | Clonality | References | Details |
---|---|---|---|---|---|---|---|---|---|
13.082039 | ABIN3003456 | IF/ICC IHC WB | Rabbit | IgG | Polyclonal | 0 | |||
10.082039 | ABIN1049140 | IHC IHC (p) | Rabbit | Modulating Domain | Polyclonal | 0 | |||
10.082039 | ABIN1049142 | IHC IHC (p) | Rabbit | C-Term | Polyclonal | 0 | |||
10 | ABIN2451820 | IF IHC IHC (p) WB | Rabbit | AA 175-205 | Polyclonal | 0 | |||
7.9152493 | ABIN4340623 | IHC IHC (p) WB | Mouse | IgG1 | 5C2 | 0 | |||
7.0820394 | ABIN6755438 | IHC IHC (p) WB | Rabbit | IgG | AA 351-400 | Polyclonal | 0 | ||
5.5 | ABIN2727595 | IHC WB | Mouse | IgG1 | AA 332-626 | OTI2E1 | 1 | ||
1 | ABIN2626154 | IHC IHC (p) WB | Mouse | IgG1 | AA 332-626 | 2A6 | 0 | ||
1 | ABIN2626153 | IHC IHC (p) WB | Mouse | IgG1 | AA 332-626 | 2B11 | 0 | ||
1 | ABIN2626155 | IHC IHC (p) WB | Mouse | IgG1 | AA 332-626 | 2E1 | 0 | ||
1 | ABIN2629009 | IHC IHC (p) WB | Rabbit | IgG | AA 612-631 | Polyclonal | 0 | ||
1 | ABIN1941185 | IHC ELISA WB | PE | Rabbit | IgG | AA 175-205 | Polyclonal | 0 | |
1 | ABIN1941182 | IHC ELISA WB | APC | Rabbit | IgG | AA 175-205 | Polyclonal | 0 | |
1 | ABIN1941184 | IHC ELISA WB | FITC | Rabbit | IgG | AA 175-205 | Polyclonal | 0 | |
1 | ABIN1941181 | IHC ELISA WB | Alkaline Phosphatase (AP) | Rabbit | IgG | AA 175-205 | Polyclonal | 0 | |
1 | ABIN2573760 | ELISA IHC WB | Rabbit | IgG | AA 175-205 | Polyclonal | 0 | ||
1 | ABIN2373149 | IHC WB | Rabbit | IgG | C-Term | Polyclonal | 0 | ||
1 | ABIN1941186 | IHC ELISA WB | HRP | Rabbit | IgG | AA 175-205 | Polyclonal | 0 | |
1 | ABIN1941183 | IHC ELISA WB | Biotin | Rabbit | IgG | AA 175-205 | Polyclonal | 0 | |
1 | ABIN2373155 | IHC ELISA WB | Rabbit | IgG | Center, Internal Region | Polyclonal | 0 |
General |
|
---|---|
Antigen | Nuclear Receptor Subfamily 4, Group A, Member 3 (NR4A3) Antikörper |
Epitop | Internal Region Alternativen |
Reaktivität | Rind (Kuh), Hund, Human, Schwein, Kaninchen Alternativen |
Wirt | Kaninchen Alternativen |
Klonalität | |
Konjugat | Unkonjugiert Alternativen |
Applikation |
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Alternativen
|
Hersteller | |
ProduktdetailsAntigendetails Anwendungsinformationen Handhabung Bilder |
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Marke | IHC-plus™ |
Spezifität | Human NR4A3. BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
Homologie | Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bovine, Dog, Elephant, Panda, Rabbit, Pig, Platypus (100%) Marmoset, Mouse, Rat, Bat, Hamster, Opossum, Turkey, Chicken (94%). |
Reinigung | Immunoaffinity purified |
Immunogen |
Synthetic 17 amino acid peptide from internal region of human NR4A3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bovine, Dog, Elephant, Panda, Rabbit, Pig, Platypus (100%), Marmoset, Mouse, Rat, Bat, Hamster, Opossum, Turkey, Chicken (94%). Type of Immunogen: Synthetic peptide |
AntigendetailsProduktdetails Anwendungsinformationen Handhabung Bilder zurück nach oben |
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Antigen | |
Andere Bezeichnung | Nor-1 / NR4A3 (NR4A3 Antibody Abstract) |
Hintergrund |
Name/Gene ID: NR4A3 Subfamily: NR4 Nerve growth factor-like Family: NHR Synonyms: NR4A3, CHN, MINOR, NOR1, Neuron-derived orphan receptor, Nuclear hormone receptor NOR-1, Nor-1, CSMF, Orphan receptor nor1, TEC receptor |
Gen-ID | 8013 |
Pathways | Fc-epsilon Rezeptor Signalübertragung, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway |
AnwendungsinformationenProduktdetails Antigendetails Handhabung Bilder zurück nach oben |
|
Applikations-hinweise |
Approved: IHC, IHC-P (10 μg/mL) Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. |
Kommentare |
Target Species of Antibody: Human |
Testdurchführung |
The IHC-pro Immunohistochemistry Protocol Tissue Preparation Formalin fixation and embedding in paraffin wax Tissue Sectioning Make 4-µm sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60°C Deparaffinization Wash slides in 3 changes of xylene – 5 minutes each at room temperature. Rehydration Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature. Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature. Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature. Rinse slides in gentle running distilled water – 5 minutes at room temperature. Antigen retrieval Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes Remove from heat and let stand at room temperature in buffer - 20 minutes Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature. Immunostaining Do not allow tissues to dry at any time during the staining procedure. Apply a universal protein block – 20 minutes at room temperature. Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature. Rinse slides in 1X TBST - 1 minute at room temperature. Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature. Rinse slides 1X TBST – 1 minute at room temperature. Apply alkaline phosphatase streptavidin – 30 minutes at room temperature. Rinse slides in 1X TBST - 1 minute at room temperature. Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature. Wash slides in distilled water – 1 minute at room temperature. Dehydrate This method should only be used if the chromogen substrate is alcohol insoluble. Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature. Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature. Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature. Wash slides in 3 changes of xylene – 1 minute each at room temperature. Apply coverslip |
Beschränkungen | Nur für Forschungszwecke einsetzbar |
HandhabungProduktdetails Antigendetails Anwendungsinformationen Bilder zurück nach oben |
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Format | Liquid |
Konzentration | Lot specific |
Buffer | PBS, less than 0.1 % sodium azide. |
Konservierungs-mittel | Sodium azide |
Vorsichtsmaßnahmen | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Lagerung | 4 °C,-20 °C |
Informationen zur Lagerung | Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles. |
Haltbarkeit | 12 months |
BilderProduktdetails Antigendetails Anwendungsinformationen Handhabung zurück nach oben |
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Bilder des Herstellers |