Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Lambda Protein Phosphatase

400 U/ µL
Produktnummer ABIN2451909
  • Reaktivität
    Säugetier
    Wirt
    Escherichia coli (E. coli)
    Spezifität
    Release of phosphate groups from phosphorylated serine, threonine, tyrosine and histidine residues in proteins.
    Produktmerkmale
    This product is an intact enzyme of high quality without tag. Lambda Protein Phosphatase (Lambda -PPase) is a Mn+2-dependent protein phosphatase with activity towards phosphorylated serine, threonine, tyrosine and histidine residues. It is the 221 amino-acid product of ORF221 open reading frame on bacteriophage lambda. Lambda -PPase was expressed as a recombinant protein in E.coli and highly purified.
    Reinheit
    > 95 % homogeneous protein determined by SDS-PAGE (CBB staining) that contains no detectable protease activity
    Bestandteile
    10 x lambda -PPase Reaction Buffer [500m M Tris-HCl (pH 7.6), 1 M NaCl, 20 mM dithiothreitol, 1 mM EDTA, 0.1 % Brij 35]
    10 x Mn2+ (20 mM MnCl2)
  • Applikationshinweise
    Lambda -PPase can be used to release phosphate groups from phosphorylated serine, threonine, tyrosine and histidine residues in proteins. It should be noted that different proteins are dephosphorylated at different rates. Optimal reaction temperature is 30 °C. Inclusion of protease inhibitor cocktail and shortest incubation time is desired when assays are done with crude samples.
    Kommentare

    Specific Activity: 400,000 U/mg
    One unit is defined as the amount of enzyme that hydrolyzes 1 nmole of p-nitrophenyl phosphate per minute at 30 °C. Unit definition assays are performed with 50 mM p-nitrophenyl phosphate in Lambda-PPase buffer, supplemented with 2 mM MnCl2 in a 50

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    400 U/ µL
    Buffer
    50 mM HEPES (pH 7.5), 100 mM NaCl, 2 mM dithiothreitol, 0.1 mM MnCl2,0.1 mM EDTA, 50 % glycerol, 0.01 % Brij 35.
    Konservierungsmittel
    Dithiothreitol (DTT)
    Vorsichtsmaßnahmen
    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Aliquote to avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
  • UniProt
    P03772
Sie sind hier:
Kundenservice