anti-Human MBD4 Antikörper für Western Blotting

Recommended MBD4 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Methyl-CpG Binding Domain Protein 4 (MBD4) Antikörper
  • MED1
  • Med1
  • methyl-CpG binding domain protein 4
  • MBD4
  • Mbd4
Epitop
Middle Region
27
16
13
11
7
7
2
2
1
1
1
1
Reaktivität
Meerschweinchen, Human
66
11
4
Wirt
Kaninchen
66
2
1
Klonalität
Polyklonal
Konjugat
Dieser MBD4 Antikörper ist unkonjugiert
5
5
5
4
4
4
1
1
1
1
1
1
1
1
Applikation
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
48
35
27
14
13
12
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN2776028
$ 289.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.1469584 ABIN1881532 WB Rabbit AA 323-351, Center Anmelden zum Anzeigen Polyclonal 3
3.1469584 ABIN3044062 WB Rabbit IgG AA 566-580, C-Term Anmelden zum Anzeigen Polyclonal
3.1469584 ABIN4332947 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 2
3.1469584 ABIN2668788 WB Rabbit IgG AA 268-282 Anmelden zum Anzeigen Polyclonal
3.1469584 ABIN2682330 IP WB Rabbit IgG Center Anmelden zum Anzeigen Polyclonal
3.1469584 ABIN3031772 WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN809479 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN5535114 WB Rabbit Ig Fraction AA 323-351 Anmelden zum Anzeigen Polyclonal
1 ABIN2309983 ELISA WB FITC Rabbit IgG AA 322-351, Center, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN2309907 ELISA WB HRP Rabbit IgG AA 322-351, Center, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN2309976 ELISA WB APC Rabbit IgG AA 322-351, Center, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN2309957 ELISA WB Rabbit IgG AA 322-351, Center, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN2309886 WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2309910 ELISA WB PE Rabbit IgG AA 322-351, Center, Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN4965415 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN5516287 WB Rabbit Middle Region Anmelden zum Anzeigen Polyclonal
1 ABIN1088575 ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1088576 ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN2309944 ELISA WB APC Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2309950 ELISA WB FITC Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal

General

Antigen Methyl-CpG Binding Domain Protein 4 (MBD4) Antikörper
Epitop Middle Region
(27), (16), (13), (11), (7), (7), (2), (2), (1), (1), (1), (1)
Reaktivität Meerschweinchen, Human
(66), (11), (4)
Wirt Kaninchen
(66), (2), (1)
Klonalität
Polyklonal
Konjugat Dieser MBD4 Antikörper ist unkonjugiert
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
(48), (35), (27), (14), (13), (12), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MBD4 Antikörper

Target Details MBD4 Anwendungsinformationen Handhabung Bilder
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN979870.
Sequenz CSEQKTSGII NKFCSAKDSE HNEKYEDTFL ESEEIGTKVE VVERKEHLHT
Homologie Guinea Pig: 83%, Human: 100%
Produktmerkmale This is a rabbit polyclonal antibody against MBD4. It was validated on Western Blot using a cell lysate as a positive control.
Reinigung Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human MBD4

Target Details MBD4

Produktdetails anti-MBD4 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung MBD4 (MBD4 Antibody Abstract)
Hintergrund MBD4 is involved with DNA methylation. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD3 comprise a family of nuclear proteins related by??he presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MED1
Protein Interaction Partner: KDM1A, SUV39H1, HTT, FAS, SUMO2, UBC, CDCA2, Trim27, FADD, MLH1, CSNK2B, DNMT3B, SIN3A, HDAC1,
Protein Size: 580
Molekulargewicht 66 kDa
Gen-ID 8930
NCBI Accession NM_003925, NP_003916
UniProt O95243
Forschungsgebiet Chromatin and Nuclear Signaling
Pathways DNA Reparatur

Anwendungsinformationen

Produktdetails anti-MBD4 Antikörper Target Details MBD4 Handhabung Bilder zurück nach oben
Applikationshinweise Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

Antigen size: 580 AA

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MBD4 Antikörper Target Details MBD4 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Bilder

Produktdetails anti-MBD4 Antikörper Target Details MBD4 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Methyl-CpG Binding Domain Protein 4 (MBD4) (Middle Region) antibody (ABIN2776028) WB Suggested Anti-MBD4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Chromatin Immunoprecipitation (ChIP) image for anti-Methyl-CpG Binding Domain Protein 4 (MBD4) (Middle Region) antibody (ABIN2776028) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three ...