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Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validierenRelevance Score | ABIN | Application | Konjugat | Host | Isotype | Epitope | Hersteller | Clonality | References | Details |
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1 | ABIN2628343 | IHC (fro) IHC (p) WB | Rabbit | IgG | AA 519-537 | Anmelden zum Anzeigen | Polyclonal | 0 | ||
1 | ABIN3032662 | IHC (fro) IHC (p) WB | Rabbit | IgG | C-Term | Anmelden zum Anzeigen | Polyclonal | 0 |
General |
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Antigen | Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) Antikörper |
Epitop | C-Term, AA 500-542 Alternativen |
Reaktivität | Human, Maus, Ratte (Rattus) Alternativen |
Wirt | Kaninchen Alternativen |
Klonalität | |
Konjugat | Unkonjugiert Alternativen |
Applikation |
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Alternativen
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10 Publikationen vorhanden |
Hersteller | Anmelden zum Anzeigen |
ProduktdetailsAntigendetails Anwendungsinformationen Handhabung Referenzen Bilder |
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Reinigung | Immunogen affinity purified |
Immunogen | A synthetic peptide made to a C-terminal portion of the rat SLC1A3 protein (between residues 500-542) [UniProt P24942] |
AntigendetailsProduktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben |
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Antigen | |
Andere Bezeichnung | SLC1A3 / EAAT1 (SLC1A3 Antibody Abstract) |
Hintergrund | Gene Symbol: SLC1A3 |
Molekulargewicht | Theoretical MW: 60 kDa |
Gen-ID | 6507 |
UniProt | P24942 |
Pathways | Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport |
AnwendungsinformationenProduktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben |
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Applikationshinweise | Western Blot 2 μg/mL, Flow Cytometry 1:200-1:500, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:500, Immunohistochemistry-Frozen 1:10-1:500, Flow (Intracellular) 1:500The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Kommentare |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Protokoll |
Western blot Protocol for SLC1A3 antibody Western Blot Protocol: 1. Perform SDS-PAGE on protein samples to be analyzed, loading 10-40 µg of total protein per lane. . Electro-blot the proteins to a suitable membrane (PVDF or Nitrocellulose) according to the instructions provided by the manufacturer of the membrane and transfer apparatus. . Stain the membrane with Ponceau S (or a similar product) to assess transfer success. Mark molecular weight standards where appropriate. . Thoroughly rinse the membrane of stain with TBST. . Incubate the membrane in blocking buffer (5 % non-fat milk in TBST or 5 % BSA in TBST) as appropriate, for 60 minutes. . Dilute the SLC1A3 primary antibody as appropriate in blocking buffer and incubate for 60 minute at room temperature to overnight at 4 degrees C with gently shaking. . Wash the membrane in TBST three times for 10 minutes each. . Incubate the membrane in the appropriate secondary antibody prepared in blocking buffer (as per manufacturer's instructions) and incubate for 60 minutes at room temperature. . Wash the membrane in TBST three times for 10 minutes each (this step can be repeated as required to reduce background). . Incubate the membrane in the appropriate detection reagent in accordance with the manufacturer's instructions and image the blot.Note: Tween-20 can be added to the blocking, wash and antibody dilution buffers to a final concentration of 0.05-0.1 %.Immunohistochemistry-Paraffin protocol for SLC1A3 antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6. 0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining: . Wash sections in deionized water three times for 5 minutes each. . Wash sections in wash buffer for 5 minutes. . Block each section with 100-400 µL blocking solution for 60 minutes at room temperature. . Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 degrees C. . Remove antibody solution and wash sections in wash buffer three times for 5 minutes each. . Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature. . Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each. . Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature. . Wash sections three times in wash buffer for 5 minutes each. . Add 100-400 µL DAB substrate to each section and monitor staining closely. . As soon as the sections develop, immerse slides in deionized water. . Counterstain sections in hematoxylin. . Wash sections in deionized water two times for 5 minutes each. . Dehydrate sections. . Mount coverslips. |
Beschränkungen | Nur für Forschungszwecke einsetzbar |
HandhabungProduktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben |
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Format | Liquid |
Konzentration | 1.0 mg/mL |
Buffer |
PBS Buffer contains: 0.02 % Sodium Azide |
Konservierungsmittel | Sodium azide |
Vorsichtsmaßnahmen | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handhabung | Avoid freeze-thaw cycles |
Lagerung | 4 °C,-20 °C |
Informationen zur Lagerung | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
ReferenzenProduktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben |
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Produkt verwendet in: |
Arnold, Salvatore: "Exercise-Mediated Increase in Nigral Tyrosine Hydroxylase Is Accompanied by Increased Nigral GFR-α1 and EAAC1 Expression in Aging Rats." in: ACS chemical neuroscience, Vol. 7, Issue 2, pp. 227-39, 2016 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting Pinner, Tucholski, Haroutunian, McCullumsmith, Meador-Woodruff: "Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia." in: Schizophrenia research, Vol. 177, Issue 1-3, pp. 78-87, 2016 (Probematerial (Species): Human). Weitere Details: Western Blotting Reyes-Aguirre, Lamas: "Oct4 Methylation-Mediated Silencing As an Epigenetic Barrier Preventing Müller Glia Dedifferentiation in a Murine Model of Retinal Injury." in: Frontiers in neuroscience, Vol. 10, pp. 523, 2016 (Probematerial (Species): Human). Chotibut, Davis, Arnold, Frenchek, Gurwara, Bondada, Geddes, Salvatore: "Ceftriaxone increases glutamate uptake and reduces striatal tyrosine hydroxylase loss in 6-OHDA Parkinson's model." in: Molecular neurobiology, Vol. 49, Issue 3, pp. 1282-92, 2014 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting Vollbrecht, Simmler, Blakely, Deutch: "Dopamine denervation of the prefrontal cortex increases expression of the astrocytic glutamate transporter GLT-1." in: Journal of neurochemistry, Vol. 130, Issue 1, pp. 109-14, 2014 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting Hu, Takano, Xiang, Gilkes, Luo, Semenza: "Hypoxia-inducible factors enhance glutamate signaling in cancer cells." in: Oncotarget, Vol. 5, Issue 19, pp. 8853-68, 2014 (Probematerial (Species): Human). Weitere Details: Western Blotting Salvatore, Davis, Arnold, Chotibut: "Transient striatal GLT-1 blockade increases EAAC1 expression, glutamate reuptake, and decreases tyrosine hydroxylase phosphorylation at ser(19)." in: Experimental neurology, Vol. 234, Issue 2, pp. 428-36, 2012 Kobayashi, Millhorn: "Hypoxia regulates glutamate metabolism and membrane transport in rat PC12 cells." in: Journal of neurochemistry, Vol. 76, Issue 6, pp. 1935-48, 2001 |
Allgemeine Veröffentlichungen |
Schmitt, Asan, Pueschel, Kugler: "Cellular and regional distribution of the glutamate transporter GLAST in the CNS of rats: nonradioactive in situ hybridization and comparative immunocytochemistry." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 1, pp. 1-10, 1997 Rothstein, Dykes-Hoberg, Pardo, Bristol, Jin, Kuncl, Kanai, Hediger, Wang, Schielke, Welty: "Knockout of glutamate transporters reveals a major role for astroglial transport in excitotoxicity and clearance of glutamate." in: Neuron, Vol. 16, Issue 3, pp. 675-86, 1996 |
BilderProduktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben |
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Bilder des Herstellers |
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