anti-Human Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 Antikörper für Immunohistochemistry (Frozen Sections)

Recommended Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) Antikörper
  • AF001784
  • CG3747
  • Dglt-1
  • Dglut-1
  • Dmel\\CG3747
  • EAAT
  • EAAT1
  • dEAAT
  • dEAAT1
  • dEaat1
  • slc1a3
  • wu:fb98e05
  • wu:fd50b05
  • zgc:56661
  • zgc:77768
  • SLC1A3
  • GLAST
  • GluT-1
  • EA6
  • GLAST1
  • AI504299
  • B430115D02Rik
  • Eaat1
  • GLAST-1
  • GLU-T
  • Gmt1
  • MGluT1
  • Excitatory amino acid transporter 1
  • solute carrier family 1 member 3
  • excitatory amino acid transporter 1
  • solute carrier family 1 (glial high affinity glutamate transporter), member 3a
  • solute carrier family 1 (glial high affinity glutamate transporter), member 3
  • Eaat1
  • SLC1A3
  • PTRG_00379
  • Tsp_02392
  • slc1a3a
  • Slc1a3
Epitop
C-Term, AA 500-542
41
15
12
10
7
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
102
58
47
16
4
2
1
1
1
Wirt
Kaninchen
82
30
10
Klonalität
Polyklonal
Konjugat
Unkonjugiert
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
96
49
29
18
14
13
5
4
4
3
3
1
1
Hersteller
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Pubmed
10 Publikationen vorhanden

Bilder

Flow Cytometry (FACS) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381)
Immunohistochemistry (IHC) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381)
Western Blotting (WB) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381)
image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381)
image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381)
image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381)
Produktnummer ABIN151381
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN2628343 IHC IHC (fro) IHC (p) WB Rabbit IgG AA 519-537 Anmelden zum Anzeigen Polyclonal 0
1 ABIN3032662 IHC (fro) IHC (p) WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 0

General

Antigen Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) Antikörper
Epitop C-Term, AA 500-542
(41), (15), (12), (10), (7), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(102), (58), (47), (16), (4), (2), (1), (1), (1)
Wirt Kaninchen
(82), (30), (10)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(96), (49), (29), (18), (14), (13), (5), (4), (4), (3), (3), (1), (1)
Pubmed 10 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Reinigung Immunogen affinity purified
Immunogen A synthetic peptide made to a C-terminal portion of the rat SLC1A3 protein (between residues 500-542) [UniProt P24942]

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung SLC1A3 / EAAT1 (SLC1A3 Antibody Abstract)
Hintergrund Gene Symbol: SLC1A3
Molekulargewicht Theoretical MW: 60 kDa
Gen-ID 6507
UniProt P24942
Pathways Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikations-hinweise Western Blot 2 μg/mL, Flow Cytometry 1:200-1:500, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:500, Immunohistochemistry-Frozen 1:10-1:500, Flow (Intracellular) 1:500The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protokoll Western blot Protocol for SLC1A3 antibody
Western Blot Protocol:
1. Perform SDS-PAGE on protein samples to be analyzed, loading 10-40 µg of total protein per lane.
. Electro-blot the proteins to a suitable membrane (PVDF or Nitrocellulose) according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain the membrane with Ponceau S (or a similar product) to assess transfer success. Mark molecular weight standards where appropriate.
. Thoroughly rinse the membrane of stain with TBST.
. Incubate the membrane in blocking buffer (5 % non-fat milk in TBST or 5 % BSA in TBST) as appropriate, for 60 minutes.
. Dilute the SLC1A3 primary antibody as appropriate in blocking buffer and incubate for 60 minute at room temperature to overnight at 4 degrees C with gently shaking.
. Wash the membrane in TBST three times for 10 minutes each.
. Incubate the membrane in the appropriate secondary antibody prepared in blocking buffer (as per manufacturer's instructions) and incubate for 60 minutes at room temperature.
. Wash the membrane in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
. Incubate the membrane in the appropriate detection reagent in accordance with the manufacturer's instructions and image the blot.Note: Tween-20 can be added to the blocking, wash and antibody dilution buffers to a final concentration of 0.05-0.1 %.Immunohistochemistry-Paraffin protocol for SLC1A3 antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 60 minutes at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 degrees C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Konzentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Arnold, Salvatore: "Exercise-Mediated Increase in Nigral Tyrosine Hydroxylase Is Accompanied by Increased Nigral GFR-α1 and EAAC1 Expression in Aging Rats." in: ACS chemical neuroscience, Vol. 7, Issue 2, pp. 227-39, 2016 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Pinner, Tucholski, Haroutunian, McCullumsmith, Meador-Woodruff: "Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia." in: Schizophrenia research, Vol. 177, Issue 1-3, pp. 78-87, 2016 (Probematerial (Species): Human). Weitere Details: Western Blotting

Reyes-Aguirre, Lamas: "Oct4 Methylation-Mediated Silencing As an Epigenetic Barrier Preventing Müller Glia Dedifferentiation in a Murine Model of Retinal Injury." in: Frontiers in neuroscience, Vol. 10, pp. 523, 2016 (Probematerial (Species): Human).

Chotibut, Davis, Arnold, Frenchek, Gurwara, Bondada, Geddes, Salvatore: "Ceftriaxone increases glutamate uptake and reduces striatal tyrosine hydroxylase loss in 6-OHDA Parkinson's model." in: Molecular neurobiology, Vol. 49, Issue 3, pp. 1282-92, 2014 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Vollbrecht, Simmler, Blakely, Deutch: "Dopamine denervation of the prefrontal cortex increases expression of the astrocytic glutamate transporter GLT-1." in: Journal of neurochemistry, Vol. 130, Issue 1, pp. 109-14, 2014 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Hu, Takano, Xiang, Gilkes, Luo, Semenza: "Hypoxia-inducible factors enhance glutamate signaling in cancer cells." in: Oncotarget, Vol. 5, Issue 19, pp. 8853-68, 2014 (Probematerial (Species): Human). Weitere Details: Western Blotting

Salvatore, Davis, Arnold, Chotibut: "Transient striatal GLT-1 blockade increases EAAC1 expression, glutamate reuptake, and decreases tyrosine hydroxylase phosphorylation at ser(19)." in: Experimental neurology, Vol. 234, Issue 2, pp. 428-36, 2012

Kobayashi, Millhorn: "Hypoxia regulates glutamate metabolism and membrane transport in rat PC12 cells." in: Journal of neurochemistry, Vol. 76, Issue 6, pp. 1935-48, 2001

Allgemeine Veröffentlichungen

Schmitt, Asan, Pueschel, Kugler: "Cellular and regional distribution of the glutamate transporter GLAST in the CNS of rats: nonradioactive in situ hybridization and comparative immunocytochemistry." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 1, pp. 1-10, 1997

Rothstein, Dykes-Hoberg, Pardo, Bristol, Jin, Kuncl, Kanai, Hediger, Wang, Schielke, Welty: "Knockout of glutamate transporters reveals a major role for astroglial transport in excitotoxicity and clearance of glutamate." in: Neuron, Vol. 16, Issue 3, pp. 675-86, 1996

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody
Immunohistochemistry (IHC) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (Image 2)
Western Blotting (WB) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (Image 3)
image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [ABIN151381] - Intracellular stai...
image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [ABIN151381] - Staining of HEK293...
image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [ABIN151381] - Analysis using the...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [ABIN151381] - IHC-P ana...
Western Blotting (WB) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody [ABIN151381] - Western blot analysis of S...
Immunofluorescence (IF) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [ABIN151381] - ...
Flow Cytometry (FACS) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - An intracellular stain was performed...
Flow Cytometry (FACS) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - An intracellular stain was performed...