anti-Rind (Kuh) WNT9B Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended WNT9B Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Wingless-Type MMTV Integration Site Family, Member 9B (WNT9B) Antikörper
  • WNT14B
  • WNT15
  • WNT9B
  • wnt-9b
  • Wnt14b
  • Wnt15
  • clf
  • clf1
  • wnt-14b
  • wnt-15
  • Wnt family member 9B
  • wingless-type MMTV integration site family member 9B L homeolog
  • protein Wnt-9b
  • wingless-type MMTV integration site family, member 9B
  • WNT9B
  • wnt9b.2.L
  • Wnt9b
  • LOC468296
  • wnt9b
Epitop
Internal Region
9
4
3
2
2
2
1
1
1
1
Reaktivität
Rind (Kuh), Hamster, Pferd, Human, Affe, Maus, Schwein, Ratte (Rattus)
49
12
4
2
2
2
2
2
2
1
Wirt
Kaninchen
30
17
2
1
Klonalität
Polyklonal
Konjugat
Dieser WNT9B Antikörper ist unkonjugiert
2
2
2
1
1
1
Applikation
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
34
27
13
11
2
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN1049489
$ 484.00
Zzgl. Versandkosten $45.00

General

Antigen Wingless-Type MMTV Integration Site Family, Member 9B (WNT9B) Antikörper
Epitop Internal Region
(9), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reaktivität Rind (Kuh), Hamster, Pferd, Human, Affe, Maus, Schwein, Ratte (Rattus)
(49), (12), (4), (2), (2), (2), (2), (2), (2), (1)
Wirt Kaninchen
(30), (17), (2), (1)
Klonalität
Polyklonal
Konjugat Dieser WNT9B Antikörper ist unkonjugiert
(2), (2), (2), (1), (1), (1)
Applikation Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(34), (27), (13), (11), (2), (2), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-WNT9B Antikörper

Target Details WNT9B Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human WNT9B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig (100%) Bat, Rabbit (94%) Dog (88%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from internal region of human WNT9B. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig (100%), Bat, Rabbit (94%), Dog (88%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details WNT9B

Produktdetails anti-WNT9B Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung WNT9B / WNT15 (WNT9B Antibody Abstract)
Hintergrund Name/Gene ID: WNT9B
Family: WNT

Synonyms: WNT9B, WNT14B, WNT15, Protein Wnt-14b, Protein Wnt-15, Protein Wnt-9b
Gen-ID 7484
Pathways WNT Signalweg, Tube Formation

Anwendungsinformationen

Produktdetails anti-WNT9B Antikörper Target Details WNT9B Handhabung Bilder zurück nach oben
Applikations-hinweise Approved: IHC, IHC-P (10 μg/mL)
Kommentare

Target Species of Antibody: Human

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-WNT9B Antikörper Target Details WNT9B Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-WNT9B Antikörper Target Details WNT9B Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 9B (WNT9B) (Internal Region) antibody (ABIN1049489) Anti-WNT9B antibody ABIN1049489 IHC staining of human fetal kidney. Immunohistochemi...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 9B (WNT9B) (Internal Region) antibody (ABIN1049489) Anti-WNT9B antibody IHC of human fetal kidney. Immunohistochemistry of formalin-fixed...