Maus CCL5 ELISA Kit

Recommended CCL5 Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kits
  • Rantes
  • Scya5
  • D17S136E
  • RANTES
  • SCYA5
  • SIS-delta
  • SISd
  • TCP228
  • eoCP
  • MuRantes
  • C-C motif chemokine 5-like
  • C-C motif chemokine ligand 5
  • chemokine (C-C motif) ligand 5
  • LOC100230935
  • CCL5
  • Ccl5
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
31
27
23
9
8
6
5
4
2
1
1
1
1
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
1-200 pg/mL
Untere Nachweisgrenze
1 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN625174
$ 476.30
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.08412 ABIN415458 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78 pg/mL - 5000 pg/mL 78 pg/mL Anmelden zum Anzeigen 1
10.08412 ABIN366415 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Anmelden zum Anzeigen
10.08412 ABIN921072 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate) 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen 2
10.08412 ABIN491818 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 13.7 pg/mL - 10000 pg/mL 13.7 pg/mL Anmelden zum Anzeigen
10.08412 ABIN1029726 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Anmelden zum Anzeigen
10.08412 ABIN924828 Sandwich ELISA Cell Lysate, Serum, Plasma 16-2000 pg/mL 16 pg/mL Anmelden zum Anzeigen
10.08412 ABIN3198413 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.63-1000 pg/mL 15.63 pg/mL Anmelden zum Anzeigen
10.08412 ABIN1888536 Sandwich ELISA Plasma, Cell Culture Cells 1-200 pg/mL 1 pg/mL Anmelden zum Anzeigen
10.08412 ABIN2708435 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
10.08412 ABIN2113385 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin - citrate) Anmelden zum Anzeigen
1 ABIN5593073 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 39-2500 pg/mL 39 pg/mL Anmelden zum Anzeigen
1 ABIN3032837 Competition ELISA Cell Culture Supernatant, Plasma, Serum 0.82-200 pg/mL 0.82 pg/mL Anmelden zum Anzeigen
1 ABIN511934 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 2.5-50 ng/mL 2.5 ng/mL Anmelden zum Anzeigen
1 ABIN456345 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 39-2500 pg/mL 39 pg/mL Anmelden zum Anzeigen
1 ABIN5652807 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.82 pg/mL - 200 pg/mL 0.82 pg/mL Anmelden zum Anzeigen
1 ABIN5522264 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN2113211 Cell Lysate, Serum, Plasma 16-2000 pg/mL 16 pg/mL Anmelden zum Anzeigen
1 ABIN2538565 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.13-5000 pg/mL 78.13 pg/mL Anmelden zum Anzeigen
1 ABIN2042650 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen
1 ABIN2683689 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Anmelden zum Anzeigen

General

Antigen Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kits
Reaktivität Maus
Kits mit alternativen Reaktivitäten:
(31), (27), (23), (9), (8), (6), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 1-200 pg/mL
Untere Nachweisgrenze 1 pg/mL
Applikation ELISA
Pubmed 4 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails CCL5 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Referenzen für CCL5 Kit (ABIN625174) Bilder
Verwendungszweck Mouse RANTES (CCL5) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben Plasma, Cell Culture Supernatant, Serum
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sensitivität 1 pg/mL
Produktmerkmale
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Bestandteile
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Benötigtes Material
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Antigendetails

Produktdetails CCL5 ELISA Kit Anwendungsinformationen Handhabung Referenzen für CCL5 Kit (ABIN625174) Bilder zurück nach oben
Antigen
Andere Bezeichnung RANTES / CCL5 (CCL5 ELISA Kit Abstract)
Hintergrund C-C motif chemokine 5 (MuRantes) (SIS-delta) (Small-inducible cytokine A5) (T-cell-specific protein RANTES)
Gen-ID 20304
UniProt P30882
Forschungsgebiet Chemokines, Immunology, Innate Immunity
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration

Anwendungsinformationen

Produktdetails CCL5 ELISA Kit Antigendetails Handhabung Referenzen für CCL5 Kit (ABIN625174) Bilder zurück nach oben
Applikationshinweise Recommended Dilution for serum and plasma samples3 - 30 fold
Probenmenge 100 μL
Plattentyp Pre-coated
Protokoll
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Aufbereitung der Reagenzien 1. Bring all reagents and samples to room temperature (18 - 25°C) before use. 2. Sample dilution: Assay Diluent A (Item D) is used for dilution of serum/plasma samples, Assay Diluent C (Item L) can be used for dilution of cell culture supernates. 3. Assay Diluent B should be diluted 5-fold with deionized or distilled water before use. 4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µl Assay Diluent A (for serum/plasma samples) or Assay Diluent C (for cell culture supernates) into Item C vial to prepare a 50 ng/ml standard. Dissolve the powder thoroughly by a gentle mix. Add 4 µl RANTES standard (50 ng/ml) from the vial of Item C, into a tube with 996 µl Assay Diluent A or Assay Diluent C to prepare a 200 pg/ml standard solution. Pipette 300 µl Assay Diluent A or Assay Diluent C into each tube. Use the 200 pg/ml standard solution to produce a dilution series (shown below). Mix each tube thoroughly before the next transfer. Gently vortex to mix. Assay Diluent A or Assay Diluent C serves as the zero standard (0 pg/ml). 5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer. 6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µl of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4°C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure. 7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 10,000-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 2 µl of HRP-Streptavidin concentrate into a tube with 198 µl 1x Assay Diluent B to prepare a 100-fold diluted HRP- Streptavidin solution (do not store the diluted solution for next day use). Mix through and then pipette 100 µl of prepared 100-fold diluted solution into a tube with 10 ml 1x Assay Diluent B to prepare a final 10,000 fold diluted HRP-Streptavidin solution.
Testdurchführung 1. Bring all reagents and samples to room temperature (18 - 25°C) before use. It is recommended that all standards and samples be run at least in duplicate. 2. Add 100 µl of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4°C with gentle shaking. 3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 µl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. 4. Add 100 µl of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking. 5. Discard the solution. Repeat the wash as in step 3. 6. Add 100 µl of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking. 7. Discard the solution. Repeat the wash as in step 3. 8. Add 100 µl of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking. 9. Add 50 µl of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Ergebnisberechnung Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails CCL5 ELISA Kit Antigendetails Anwendungsinformationen Referenzen für CCL5 Kit (ABIN625174) Bilder zurück nach oben
Lagerung -20 °C
Informationen zur Lagerung The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit 6 months

Referenzen für CCL5 Kit (ABIN625174)

Produktdetails CCL5 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Tilgner, von Trotha, Gombert, Jacobs, Drechsler, Döring, Soehnlein, Grommes: "Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury." in: PLoS ONE, Vol. 11, Issue 9, pp. e0161218, 2016

Escudero, Martinez de Marañón, Collado, Gonzalez-Navarro, Hermenegildo, Peiró, Piqueras, Sanz et al.: "Combined sub-optimal doses of rosuvastatin and bexarotene impair angiotensin II-induced arterial mononuclear cell adhesion through inhibition of Nox5 signaling pathways and increased RXR/PPAR? and ..." in: Antioxidants & redox signaling, Vol. 22, Issue 11, pp. 901-20, 2015

Koppe, Högner, Doehn, Müller, Witzenrath, Gutbier, Bauer, Pribyl, Hammerschmidt, Lohmeyer, Suttorp, Herold, Opitz et al.: "Streptococcus pneumoniae stimulates a STING- and IFN regulatory factor 3-dependent type I IFN production in macrophages, which regulates RANTES production in macrophages, cocultured alveolar ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 2, pp. 811-7, 2012

Ko, Linfert, Jang, Higbee, Watkins, Cheadle, Liu, Racusen, Grigoryev, Rabb: "Transcriptional analysis of infiltrating T cells in kidney ischemia-reperfusion injury reveals a pathophysiological role for CCR5." in: American journal of physiology. Renal physiology, Vol. 302, Issue 6, pp. F762-73, 2012

Bilder

Produktdetails CCL5 ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für CCL5 Kit (ABIN625174) zurück nach oben
Bilder des Herstellers
ELISA image for Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kit (ABIN625174) Chemokine (C-C Motif) Ligand 5 (CCL5) ELISA Kit