anti-Fledermaus GRM2 Antikörper für Western Blotting

Recommended GRM2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Glutamate Receptor, Metabotropic 2 (GRM2) Antikörper
  • GLUR2
  • GPRC1B
  • MGLUR2
  • mGlu2
  • 4930441L02Rik
  • Gprc1b
  • mGluR2
  • mGluR7
  • glutamate metabotropic receptor 2
  • glutamate receptor, metabotropic 2
  • GRM2
  • Grm2
Epitop
N-Term
25
12
8
8
6
5
4
3
3
2
2
2
2
1
1
1
1
1
Reaktivität
Fledermaus, Rind (Kuh), Hund, Hamster, Human, Maus, Schwein, Kaninchen, Ratte (Rattus)
117
43
41
11
8
6
5
5
4
3
1
1
1
1
1
1
1
1
Wirt
Kaninchen
70
30
16
3
Klonalität
Polyklonal
Konjugat
Dieser GRM2 Antikörper ist unkonjugiert
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
58
42
33
29
21
13
10
5
2
1
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1048929
$ 484.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1496054 IHC (p) ELISA WB Goat AA 915-924 Anmelden zum Anzeigen Polyclonal 0
1 ABIN293491 IHC ELISA WB Rabbit IgG AA 860-872 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Glutamate Receptor, Metabotropic 2 (GRM2) Antikörper
Epitop N-Term
(25), (12), (8), (8), (6), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reaktivität Fledermaus, Rind (Kuh), Hund, Hamster, Human, Maus, Schwein, Kaninchen, Ratte (Rattus)
(117), (43), (41), (11), (8), (6), (5), (5), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(70), (30), (16), (3)
Klonalität
Polyklonal
Konjugat Dieser GRM2 Antikörper ist unkonjugiert
(3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(58), (42), (33), (29), (21), (13), (10), (5), (2), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-GRM2 Antikörper

Target Details GRM2 Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human GRM2 / MGLUR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GRM3 (42 % ).
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Rabbit, Pig (100%) Chimpanzee, Monkey, Horse, Platypus (95%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 19 amino acid peptide from N-terminal extracellular domain of human GRM2 / MGLUR2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Rabbit, Pig (100%), Chimpanzee, Monkey, Horse, Platypus (95%).

Type of Immunogen: Synthetic peptide

Target Details GRM2

Produktdetails anti-GRM2 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung GRM2 / MGLUR2 (GRM2 Antibody Abstract)
Hintergrund Name/Gene ID: GRM2
Subfamily: Metabotropic glutamate
Family: GPCR

Synonyms: GRM2, Glutamate receptor homolog, GPRC1B, MGlu2, Metabotropic, GLUR2, MGLUR2
Gen-ID 2912
Pathways cAMP Metabolic Process, Dicarboxylic Acid Transport

Anwendungsinformationen

Produktdetails anti-GRM2 Antikörper Target Details GRM2 Handhabung Bilder zurück nach oben
Applikationshinweise Approved: ICC, IHC-P (5 - 10 μg/mL), WB
Kommentare

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-GRM2 Antikörper Target Details GRM2 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-GRM2 Antikörper Target Details GRM2 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Glutamate Receptor, Metabotropic 2 (GRM2) (N-Term) antibody (ABIN1048929) Anti-GRM2 / MGLUR2 antibody ABIN1048929 IHC staining of human brain, neurons and gli...