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Blotto Immunoanalytical Grade (Non-Fat Dry Milk)

BR, DB, WB
Produktnummer ABIN925606
  • Applikation
    Blocking Reagent (BR), Dot Blot (DB), Western Blotting (WB)
    Produktmerkmale
    Supplier produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to the addition of primary antibody.
  • Applikationshinweise
    Use BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk.   Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available.  BLOTTO A is a convenient general purpose blocking agent.  BLOTTO B is recommended for blocking when phospho-specific antibodies are used.  BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form (p/n TW0025).  Users should consider the addition of protease inhibitors and phosphatase inhibitors when required.  Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Restore with deionized water (or equivalent)
    Lagerung
    -20 °C
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