Human Cell Array II

Details zu Produkt Nr. ABIN6572883
Immunocytochemistry (ICC), Microarray (MA)
Verwendungszweck Human Cell Array II is provided for immunocytochemical analysis of protein and protein-protein interaction.
Spezifität Human cell lines (HL-60, Jurkat, K-562, MOLT-4, Raji and THP-1)
Produktmerkmale Human Cell Array II carries 6 different human cell lines, including HL-60, Jurkat, K-562, MOLT-4, Raji and THP-1. Cells are harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in 4 % paraformaldehyde. The cells were arrayed on a 12-well (5 mm) adhesive coated slide, with each wells surface specifically treated to enhance cellular attachment and to minimize background staining. Each well loaded with approximately 7.5 x 103 cells. A follow-up heat dehydration process was done to ensure the attachment of cells and the stability of cellular proteins.
Fixation: 4 % paraformaldehyde
Tissue or Cell type HL-60, Jurkat, K-562, MOLT-4, Raji, THP-1
Applikations-hinweise Pretreatment with 0.1 % TritonX-100 for 1 minute at room temperature before applying immunocytochemistry is strongly suggested.

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Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung 4 °C
Informationen zur Lagerung Human Cell Array II can be stored for up to 3 months at 4 °C under desiccate conditions.
Haltbarkeit 3 months