Human Cell Array II

Details zu Produkt Nr. ABIN6572883
Reaktivität
Human
Wirt
Human
Applikation
Immunocytochemistry (ICC), Microarray (MA)
Optionen
Verwendungszweck Human Cell Array II is provided for immunocytochemical analysis of protein and protein-protein interaction.
Spezifität Human cell lines (HL-60, Jurkat, K-562, MOLT-4, Raji and THP-1)
Produktmerkmale Human Cell Array II carries 6 different human cell lines, including HL-60, Jurkat, K-562, MOLT-4, Raji and THP-1. Cells are harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in 4 % paraformaldehyde. The cells were arrayed on a 12-well (5 mm) adhesive coated slide, with each wells surface specifically treated to enhance cellular attachment and to minimize background staining. Each well loaded with approximately 7.5 x 103 cells. A follow-up heat dehydration process was done to ensure the attachment of cells and the stability of cellular proteins.
Fixation: 4 % paraformaldehyde
Tissue or Cell type HL-60, Jurkat, K-562, MOLT-4, Raji, THP-1
Applikations-hinweise Pretreatment with 0.1 % TritonX-100 for 1 minute at room temperature before applying immunocytochemistry is strongly suggested.
Kommentare

We do offer a variety of different lots. Please contact us for further information about available lots.

Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung 4 °C
Informationen zur Lagerung Human Cell Array II can be stored for up to 3 months at 4 °C under desiccate conditions.
Haltbarkeit 3 months