Human Cell Array I carries 6 different human cell lines, including 293, A-431, A549, Hela S3, Hep G2 and MCF7. Cells are harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in 4 % paraformaldehyde. The cells were arrayed on a 12-well (5 mm) adhesive coated slide, with each wells surface specifically treated to enhance cellular attachment and to minimize background staining. Each well loaded with approximately 7.5 x 103 cells. A follow-up heat dehydration process was done to ensure the attachment of cells and the stability of cellular proteins.
Fixation: 4 % paraformaldehyde
Tissue or Cell type 293, A-431, A549, Hela S3, Hep G2, MCF7