anti-Maus FASL Antikörper für Flow Cytometry

Recommended FASL Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antikörper
  • ALPS1B
  • APT1LG1
  • APTL
  • CD178
  • CD95-L
  • CD95L
  • FASL
  • TNFSF6
  • fasl
  • Fas-L
  • Faslg
  • Tnfsf6
  • gld
  • Apt1Lg1
  • Fasl
  • FASLG
  • zgc:162027
  • Fas ligand
  • Fas ligand L homeolog
  • Fas ligand (TNF superfamily, member 6)
  • FASLG
  • faslg.L
  • Fasl
  • Faslg
  • faslg
Reaktivität
Human, Maus
437
174
86
36
7
5
5
3
3
2
1
Wirt
Hamster
278
171
48
33
32
9
1
Klonalität
Monoklonal
Konjugat
Dieser FASL Antikörper ist konjugiert mit Biotin
46
32
29
14
13
13
10
10
9
9
9
9
9
9
8
7
7
3
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS)
337
261
127
100
76
70
52
48
29
28
13
12
10
10
9
5
3
2
2
1
1
1
1
1
1
1
Hersteller
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Bilder

Produktnummer ABIN1105946
$ 368.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
13.842636 ABIN1105942 FACS Hamster IgG Anmelden zum Anzeigen FLIM58 14
13.842636 ABIN1105948 FACS FITC Hamster IgG Anmelden zum Anzeigen FLIM58 14
13.842636 ABIN1105951 FACS Hamster IgG AA 132-146 Anmelden zum Anzeigen FLIM4 5
13.842636 ABIN1176980 BR FACS Armenian Hamster IgG3, kappa Anmelden zum Anzeigen MFL4 11
13.842636 ABIN614573 FACS FITC Hamster IgG Anmelden zum Anzeigen MFL4 3
13.842636 ABIN614579 FACS PE Hamster IgG Anmelden zum Anzeigen MFL4 3
13.842636 ABIN2665563 CyTOF FACS IHC Hamster IgG Anmelden zum Anzeigen MFL3 9
13.842636 ABIN2663097 FACS PE Hamster IgG Anmelden zum Anzeigen MFL3 9
13.842636 ABIN2665465 FACS Hamster IgG Anmelden zum Anzeigen MFL3 9
13.842636 ABIN2660963 FACS Biotin Hamster IgG Anmelden zum Anzeigen MFL3 8
13.842636 ABIN4895785 FACS APC Rat IgG1 AA 132-279 Anmelden zum Anzeigen 101624 0
13.842636 ABIN2478831 FACS Hamster IgG Anmelden zum Anzeigen MFL4 3
13.842636 ABIN2665564 FACS IF IHC Hamster IgG Anmelden zum Anzeigen MFL4 6
13.842636 ABIN447421 FACS FITC Armenian Hamster IgG Anmelden zum Anzeigen MFL4 0
13.842636 ABIN2660964 FACS Biotin Hamster IgG Anmelden zum Anzeigen MFL4 6
13.842636 ABIN262443 FACS IHC (p) IHC Mouse IgG2b kappa Anmelden zum Anzeigen Kay-10 0
13.842636 ABIN262444 FACS Biotin Mouse IgG2b kappa Anmelden zum Anzeigen Kay-10 0
13.842636 ABIN187289 FACS IF WB Rat IgG2a Anmelden zum Anzeigen H11 0
1 ABIN2689024 FACS Biotin Armenian Hamster IgG1, kappa Anmelden zum Anzeigen MFL3 17
1 ABIN2689022 FACS Armenian Hamster IgG1, kappa Anmelden zum Anzeigen MFL3 17

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antikörper
Reaktivität Human, Maus
(437), (174), (86), (36), (7), (5), (5), (3), (3), (2), (1)
Wirt Hamster
(278), (171), (48), (33), (32), (9), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser FASL Antikörper ist konjugiert mit Biotin
(46), (32), (29), (14), (13), (13), (10), (10), (9), (9), (9), (9), (9), (9), (8), (7), (7), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Applikation Flow Cytometry (FACS)
(337), (261), (127), (100), (76), (70), (52), (48), (29), (28), (13), (12), (10), (10), (9), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 14 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-FASL Antikörper

Target Details FASL Anwendungsinformationen Handhabung Referenzen für anti-FASL Antikörper (ABIN1105946) Bilder
Spezifität This antibody reacts with mouse FasL of B6, C3H and MRL mice as well as Balb/c and DBA/1 mice, but does not detect gld-type mouse FasL mutant by flow cytometric analysis. We have tested the reactivity on flow cytometry using mouse cell line W40LFL, which is overexpressor of mouse FasL extracellular region (See ref. 11) for more information about this cell line).
Kreuzreaktivität (Details) Species reactivity (tested):Mouse. Weakly with human.
Reinigung Protein A agarose
Immunogen W40LFL cells, which is overexpressed mouse FasL extracellular region
Klon FLIM58
Isotyp IgG

Target Details FASL

Produktdetails anti-FASL Antikörper Anwendungsinformationen Handhabung Referenzen für anti-FASL Antikörper (ABIN1105946) Bilder zurück nach oben
Antigen
Andere Bezeichnung CD178 / Fas Ligand (FASL Antibody Abstract)
Hintergrund Fas ligand (FasL/CD178/CD95L) is a type II-membrane protein, whose N terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor Fas, also termed Apo-1 or CD95, is a cell-surface type I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. It is known that engagement of Fas by FasL results in apoptotic cell death. Binding of a trimeric FasL to Fas induces trimerization of Fas, and FADD/MORT1 binds to the trimerized Fas cytoplasmic region through the interaction of the respective death domains. Caspase-8 is then recruited to FADD/MORT1 through binding of each death effector domains (DED), which in turn may induce self-activation of the protease domain and eventually leads cell to apoptosis. FasL is predominantly expressed in activated T lymphocytes and Natural Killer (NK) cells, although it is expressed in the tissues of the immune-privilege sites such as the testis and eye. Furthermore, a variety of cancers such as melanomas were found to express FasL in addition to Fas. On the other hand, Fas is expressed in various tissues with abundant expression in the thymus, liver, heart, and kidney. It is reported that Fas/FasL system is involved in the deletion of activated or autoreactive T and B cells. Mature T cells of normal mice are known to die, after they accomplish their tasks. On the contrary, mature T cells from lpr and gld mice, which are deficient of Fas and FasL respectively, do not die after activation, and activated cells accumulate in the lymph nodes and spleens of these mice. Moreover T cell hybridomas activated in the presence of Fas-neutralizing molecule, do not die. In addition to T cells, the Fas-deficient mice accumulate B cells and have elevated levels of immunoglobulins (Ig) of various classes that include anti-ssDNA and anti-dsDNA antibodies. FasL can be used as an immune-suppressive agent. Rejection of transplants is mediated by activated T cells that may express functional Fas. If a transplanted tissue is engineered to express FasL or is co-transplanted with FasL expressing cells, the transplant may be tolerated. Also Fas/ FasL system has been shown to play a role in human diseases such as fulminant hepatitis, AIDS, cancers and other diseases involving CTL-induced tissue destruction.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6
Gen-ID 356
NCBI Accession NP_000630
UniProt P48023
Pathways Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails anti-FASL Antikörper Target Details FASL Handhabung Referenzen für anti-FASL Antikörper (ABIN1105946) Bilder zurück nach oben
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-FASL Antikörper Target Details FASL Anwendungsinformationen Referenzen für anti-FASL Antikörper (ABIN1105946) Bilder zurück nach oben
Konzentration 20 µg/mL
Buffer PBS, 0.09 % NaN3, 1 % BSA
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store at 2 - 8 °C.

Referenzen für anti-FASL Antikörper (ABIN1105946)

Produktdetails anti-FASL Antikörper Target Details FASL Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Imamura, Konaka, Matsumoto, Hasegawa, Fukui, Mukaida, Kinoshita, Suda: "Fas ligand induces cell-autonomous NF-kappaB activation and interleukin-8 production by a mechanism distinct from that of tumor necrosis factor-alpha." in: The Journal of biological chemistry, Vol. 279, Issue 45, pp. 46415-23, 2004

Plath, Wilkinson, Stirling, Rapoport: "Interactions between Sec complex and prepro-alpha-factor during posttranslational protein transport into the endoplasmic reticulum." in: Molecular biology of the cell, Vol. 15, Issue 1, pp. 1-10, 2003

Margalit, Fishman, Berko, Engberg, Gross: "Chimeric beta2 microglobulin/CD3zeta polypeptides expressed in T cells convert MHC class I peptide ligands into T cell activation receptors: a potential tool for specific targeting of pathogenic CD8(+) T cells." in: International immunology, Vol. 15, Issue 11, pp. 1379-87, 2003

Fukui, Imamura, Umemura, Kawabe, Suda: "Pathogen-associated molecular patterns sensitize macrophages to Fas ligand-induced apoptosis and IL-1 beta release." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 4, pp. 1868-74, 2003

Nakamoto, Kaneko, Fan, Momoi, Tsutsui, Nakanishi, Kobayashi, Suda: "Prevention of hepatocellular carcinoma development associated with chronic hepatitis by anti-fas ligand antibody therapy." in: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1105-11, 2002

Saegusa, Ishimaru, Yanagi, Mishima, Arakaki, Suda, Saito, Hayashi: "Prevention and induction of autoimmune exocrinopathy is dependent on pathogenic autoantigen cleavage in murine Sjögren's syndrome." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 2, pp. 1050-7, 2002

Roger, Chalifour, Lemieux, Duplay: "Cutting edge: Ly49A inhibits TCR/CD3-induced apoptosis and IL-2 secretion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 1, pp. 6-10, 2001

Asao, Okuyama, Kumaki, Ishii, Tsuchiya, Foster, Sugamura: "Cutting edge: the common gamma-chain is an indispensable subunit of the IL-21 receptor complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 1, pp. 1-5, 2001

Saito, Haruta, Shimuta, Inoue, Sakurai, Yamada, Ishimaru, Higashiyama, Sumida, Ishida, Suda, Noda, Hayashi, Tsubota: "Fas ligand-mediated exocrinopathy resembling Sjögren's syndrome in mice transgenic for IL-10." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 5, pp. 2488-94, 1999

Miwa, Hashimoto, Yatomi, Nakamura, Nagata, Suda: "Therapeutic effect of an anti-Fas ligand mAb on lethal graft-versus-host disease." in: International immunology, Vol. 11, Issue 6, pp. 925-31, 1999

Waring, Müllbacher: "Cell death induced by the Fas/Fas ligand pathway and its role in pathology." in: Immunology and cell biology, Vol. 77, Issue 4, pp. 312-7, 1999

Zeytun, Hassuneh, Nagarkatti, Nagarkatti: "Fas-Fas ligand-based interactions between tumor cells and tumor-specific cytotoxic T lymphocytes: a lethal two-way street." in: Blood, Vol. 90, Issue 5, pp. 1952-9, 1997

Hueber, Zörnig, Lyon, Suda, Nagata, Evan: "Requirement for the CD95 receptor-ligand pathway in c-Myc-induced apoptosis." in: Science (New York, N.Y.), Vol. 278, Issue 5341, pp. 1305-9, 1997

Kayagaki, Yamaguchi, Nagao, Matsuo, Maeda, Okumura, Yagita: "Polymorphism of murine Fas ligand that affects the biological activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3914-9, 1997

Bilder

Produktdetails anti-FASL Antikörper Target Details FASL Anwendungsinformationen Handhabung Referenzen für anti-FASL Antikörper (ABIN1105946) zurück nach oben
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (Biotin) (ABIN1105946) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (Biotin)