anti-Frosch Cytochrome C Antikörper für Western Blotting

Recommended Cytochrome C Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Cytochrome C, Somatic (CYCS) Antikörper
  • CYC
  • HCS
  • THC4
  • cyct
  • cyc
  • hcs
  • CYCS
  • cytc
  • CYCSA
  • CG17903
  • Cyt C
  • Cyt c
  • Cyt-c2
  • CytC-2
  • Cytc
  • Cytc-p
  • DC4
  • DMc01
  • Dmel\\CG17903
  • cyt c
  • cyt c-p
  • cyt-c-p
  • cyt-c2
  • cyt.c
  • cytochrome c
  • dc4
  • pDMc01
  • wu:fk52b01
  • zgc:86706
  • cytochrome c, somatic
  • cytochrome c, somatic S homeolog
  • Cytochrome c proximal
  • cytochrome c, somatic b
  • cytochrome c-like
  • cytochrome c
  • CYCS
  • cycs.S
  • cycs
  • Cycs
  • Cyt-c-p
  • cycsb
  • LOC100724240
  • CYC
  • LOC100847700
  • LOC101107954
  • LOC106829744
  • LOC106992390
Epitop
AA 1-80
28
16
13
13
8
7
6
5
4
4
4
3
3
3
1
1
1
1
Reaktivität
Hund, Frosch, Fruchtfliege (Drosophila melanogaster), Pferd, Human, Maus, Taube, Ratte (Rattus)
355
294
220
95
72
59
38
32
29
28
27
21
20
12
9
8
8
7
5
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Wirt
Maus
258
150
7
3
Klonalität
Monoklonal
Konjugat
Dieser Cytochrome C Antikörper ist unkonjugiert
19
14
11
8
8
6
6
6
6
5
5
5
5
5
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
Applikation
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
303
157
152
91
74
58
47
34
26
22
13
6
6
5
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Hersteller
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Bilder

Produktnummer ABIN3025122
$ 361.90
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN3024593 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 93-104 Anmelden zum Anzeigen SPM389 0
1 ABIN3024590 FACS IF IHC (p) WB Mouse IgG2b, kappa Anmelden zum Anzeigen CTC05 0
1 ABIN3025124 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 1-80 Anmelden zum Anzeigen 7H8-2C12 0
1 ABIN3024594 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 93-104 Anmelden zum Anzeigen SPM389 0
1 ABIN3024592 FACS IF IHC (p) WB Mouse IgG2b, kappa Anmelden zum Anzeigen CTC05 0

General

Antigen Cytochrome C, Somatic (CYCS) Antikörper
Epitop AA 1-80
(28), (16), (13), (13), (8), (7), (6), (5), (4), (4), (4), (3), (3), (3), (1), (1), (1), (1)
Reaktivität Hund, Frosch, Fruchtfliege (Drosophila melanogaster), Pferd, Human, Maus, Taube, Ratte (Rattus)
(355), (294), (220), (95), (72), (59), (38), (32), (29), (28), (27), (21), (20), (12), (9), (8), (8), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Wirt Maus
(258), (150), (7), (3)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Cytochrome C Antikörper ist unkonjugiert
(19), (14), (11), (8), (8), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(303), (157), (152), (91), (74), (58), (47), (34), (26), (22), (13), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Cytochrome C Antikörper

Target Details Cytochrome C Anwendungsinformationen Handhabung Bilder
Produktmerkmale It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Reinigung Protein G affinity chromatography
Immunogen Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon Cytochrome C was used as the immunogen for the Cytochrome C antibody.
Klon 7H8-2C12
Isotyp IgG2b, kappa

Target Details Cytochrome C

Produktdetails anti-Cytochrome C Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Cytochrome C (CYCS Antibody Abstract)
Hintergrund It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Pathways Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails anti-Cytochrome C Antikörper Target Details Cytochrome C Handhabung Bilder zurück nach oben
Applikations-hinweise Optimal dilution of the Cytochrome C antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Cytochrome C Antikörper Target Details Cytochrome C Anwendungsinformationen Bilder zurück nach oben
Konzentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store the Cytochrome C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Bilder

Produktdetails anti-Cytochrome C Antikörper Target Details Cytochrome C Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Cytochrome C, Somatic (CYCS) (AA 1-80) antibody (ABIN3025122) Formalin-fixed, paraffin-embedded human pancreas stained with Cytochrome C antibody (...
Immunohistochemistry (IHC) image for anti-Cytochrome C, Somatic (CYCS) (AA 1-80) antibody (ABIN3025122) Formalin-fixed, paraffin-embedded human salivary tumor stained with Cytochrome C anti...