anti-Human BID Antikörper für Immunofluorescence (fixed cells)

Recommended BID Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antikörper
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist S homeolog
  • BID
  • Bid
  • bida
  • bid.S
  • bid
Epitop
AA 1-195
58
37
35
19
17
15
11
10
10
8
7
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
330
172
77
9
5
3
2
Wirt
Maus
306
96
21
9
Klonalität
Monoklonal
Konjugat
Dieser BID Antikörper ist unkonjugiert
24
22
14
9
8
7
4
4
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Cell Culture (CC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC), Western Blotting (WB)
365
184
170
90
55
41
36
24
15
15
11
11
3
2
1
1
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1996893
$ 218.90
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1845950 ELISA FACS IF/ICC IHC WB Mouse IgG1 Anmelden zum Anzeigen 0
1 ABIN1996901 ELISA IF IF/ICC Rabbit IgG AA 1-195 Anmelden zum Anzeigen 005 0
1 ABIN2909790 IF/ICC IHC IP WB Rabbit AA 1-195 Anmelden zum Anzeigen Polyclonal 0
1 ABIN6054439 IF/ICC IHC WB FITC Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN6050925 IF/ICC IHC WB Biotin Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN5858675 ELISA IF/ICC IHC IP WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN6021022 IF/ICC IHC IP WB Mouse Anmelden zum Anzeigen 0

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antikörper
Epitop AA 1-195
(58), (37), (35), (19), (17), (15), (11), (10), (10), (8), (7), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(330), (172), (77), (9), (5), (3), (2)
Wirt Maus
(306), (96), (21), (9)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser BID Antikörper ist unkonjugiert
(24), (22), (14), (9), (8), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Cell Culture (CC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC), Western Blotting (WB)
(365), (184), (170), (90), (55), (41), (36), (24), (15), (15), (11), (11), (3), (2), (1), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-BID Antikörper

Target Details BID Anwendungsinformationen Handhabung Bilder
Spezifität Human BID
No cross-reactivity in ELISA with
E.coli cell lysate
Keine Kreuzreaktivität Escherichia coli (E. coli)
Produktmerkmale This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BID (rh BID, ABIN2002909 P55957-1, Met 1-Asp 195,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Reinigung purified by Protein A affinity chromatography
Sterilität 0.2 μm filtered
Immunogen Recombinant Human BID protein (ABIN2002909)
Klon 10
Isotyp IgG1
Plasmids, Primers & others

Target Details BID

Produktdetails anti-BID Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung BID (BID Antibody Abstract)
Hintergrund BID, BH3-interacting domain death agonist, is a member of the Bcl-2 protein family. BID contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. It is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent, and also offer a new strategy for the treatment of acute renal failure associated with ischemia-reperfusion. BID receives direct inputs from a key regulator of the cell cycle arrest/DNA repair machinery (ATM), and therefore is an excellent candidate to coordinate genotoxic stress responses and apoptotic cell death. BID is a novel pro-apoptosis Bcl-2 family protein that is activated by caspase 8 in response to Fas/TNF-R1 death receptor signals. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. Recent studies further indicate that BID may be more than just a killer molecule. Deletion of BID inhibits carcinogenesis in the liver, although this genetic alteration promotes tumorigenesis in the myeloid cells. This is likely related to the function of BID to promote cell cycle progression into S phase. BID could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint.
UniProt P55957-1
Pathways Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails anti-BID Antikörper Target Details BID Handhabung Bilder zurück nach oben
Applikations-hinweise WB: 2-5 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect BID. The detection limit for Human BID is approximately 0.078 ng/well.
ELISA(Cap): 0.5-4 μg/mL
ICC/IF: 10-25 μg/mL
FCM: 0.5-2 μg/Test
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-BID Antikörper Target Details BID Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Konservierungs-mittel Without preservative
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung 4 °C,-20 °C,-80 °C
Informationen zur Lagerung This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-BID Antikörper Target Details BID Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN1996893) Human BID expression in Jurkat cells. The cells were treated according to manufacture...
Western Blotting (WB) image for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN1996893) anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (Image 2)