anti-Human BAD Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended BAD Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
BCL2-Associated Agonist of Cell Death (BAD) Antikörper
  • bad
  • fa01b12
  • wu:fa01b12
  • wu:fa96d04
  • BAD
  • BBC2
  • BCL2L8
  • AI325008
  • Bbc2
  • BCL2-associated agonist of cell death b
  • BCL2 associated agonist of cell death
  • BCL2-associated agonist of cell death
  • badb
  • BAD
  • Bad
Epitop
AA 140-168
58
58
47
47
46
45
44
39
32
22
19
19
19
19
19
19
18
18
18
18
18
18
18
18
18
18
18
18
17
17
15
14
14
11
11
11
10
9
9
8
7
7
6
5
5
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
778
468
301
19
19
8
5
4
3
2
2
1
1
1
1
1
1
Wirt
Kaninchen
988
46
2
1
Klonalität
Polyklonal
Konjugat
Dieser BAD Antikörper ist unkonjugiert
67
66
66
60
60
60
6
6
6
6
6
6
6
6
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
543
538
405
347
124
78
53
37
14
13
11
10
4
3
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN674709
$ 306.90
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
9.954776 ABIN461986 IHC IHC (p) IP WB Mouse IgG N-Term Anmelden zum Anzeigen BYC001 0
9.954776 ABIN213744 IHC IHC (p) WB Rabbit IgG Ser112 Anmelden zum Anzeigen Polyclonal 0
9.954776 ABIN214418 IHC IHC (p) WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 0
9.954776 ABIN5949401 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG Anmelden zum Anzeigen SA32-01 0
9.954776 ABIN498774 IHC (p) WB Rabbit pSer112 Anmelden zum Anzeigen Polyclonal 0
9.954776 ABIN498190 IF IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal 0
9.954776 ABIN498864 IHC (p) Rabbit Anmelden zum Anzeigen Polyclonal 0
9.954776 ABIN682828 IF (p) IHC (p) WB Rabbit IgG pSer75 Anmelden zum Anzeigen Polyclonal 0
9.954776 ABIN756562 IF (p) IHC (p) WB Rabbit IgG pSer118 Anmelden zum Anzeigen Polyclonal 0
9.954776 ABIN498091 IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal 0
9.954776 ABIN4282865 IHC (p) IP WB Mouse IgG N-Term Anmelden zum Anzeigen BYC001 0
9.954776 ABIN4282864 IHC IHC (p) IP WB Rabbit Anmelden zum Anzeigen Polyclonal 0
9.954776 ABIN5557534 IHC (p) WB Rabbit IgG Anmelden zum Anzeigen 5D4 0
9.954776 ABIN4282859 ICC IF IHC IHC (p) IP WB Rabbit Anmelden zum Anzeigen Polyclonal 0
9.954776 ABIN4282868 ICC IF IHC IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN388109 IHC (p) WB Rabbit AA 53-81, Center Anmelden zum Anzeigen Polyclonal 2
1 ABIN358087 EIA IHC (p) WB Rabbit Ig Fraction pSer99 Anmelden zum Anzeigen Polyclonal 5
1 ABIN388118 FACS IHC (p) WB Rabbit Ig Fraction AA 92-127, BH3 Domain Anmelden zum Anzeigen Polyclonal 3
1 ABIN389521 DB IF IHC (p) Rabbit Ig Fraction pSer99 Anmelden zum Anzeigen Polyclonal 2
1 ABIN389519 IHC (p) WB Rabbit Ig Fraction pSer134 Anmelden zum Anzeigen Polyclonal 2

General

Antigen BCL2-Associated Agonist of Cell Death (BAD) Antikörper
Epitop AA 140-168
(58), (58), (47), (47), (46), (45), (44), (39), (32), (22), (19), (19), (19), (19), (19), (19), (18), (18), (18), (18), (18), (18), (18), (18), (18), (18), (18), (18), (17), (17), (15), (14), (14), (11), (11), (11), (10), (9), (9), (8), (7), (7), (6), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(778), (468), (301), (19), (19), (8), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(988), (46), (2), (1)
Klonalität
Polyklonal
Konjugat Dieser BAD Antikörper ist unkonjugiert
(67), (66), (66), (60), (60), (60), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(543), (538), (405), (347), (124), (78), (53), (37), (14), (13), (11), (10), (4), (3), (2), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-BAD Antikörper

Target Details BAD Anwendungsinformationen Handhabung Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human Bad
Isotyp IgG
Plasmids, Primers & others

Target Details BAD

Produktdetails anti-BAD Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Bad (BAD Antibody Abstract)
Hintergrund

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Subcellular location: Cytoplasm, Mitochondrion

Synonyms: BBC2, BCL2L8, Bcl2-associated agonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, BBC6

Gen-ID 572
UniProt Q92934
Pathways MAPK Signalweg, PI3K-Akt Signalweg, RTK Signalweg, Apoptose, Fc-epsilon Rezeptor Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events

Anwendungsinformationen

Produktdetails anti-BAD Antikörper Target Details BAD Handhabung Bilder zurück nach oben
Applikations-hinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-BAD Antikörper Target Details BAD Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Bilder

Produktdetails anti-BAD Antikörper Target Details BAD Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Lane 1:Mouse kidney lysate Lane 2: Mouse brain lysate probed with Rabbit Anti-Bad Pol...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Bad Polyclonal A...
Western Blotting (WB) image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Image provided by the Independent Validation Program (badge number 29802). Lane 1: MC...
'Independent Validation' Siegel
Chargennummer 090915
Validierte Anwendung Western Blotting
Positivkontrolle MCF7 cells
Negativkontrolle C6/36 cells (non-reactive species)
Bewertung A strong band was observed in the positive control sample at the correct molecular weight. No bands were observed in the negative control sample.
Primärantikörper
Sekundärantikörper
Kontrollen
  • Positive control: MCF7 cells
  • Negative control: C6/36 cells
Protokoll
  • 1. The cell extracts were heated at 95°C for 5 minutes in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol.
  • 2. 15 μl of heated extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 ml of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 hour.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution in TBST containing 5% (W/V) BSA and incubated for 16 hours at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 minutes each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 minutes each.
  • 11. The membrane was rinsed in TBS twice for 30 seconds each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 600 seconds.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 minutes each.
  • 15. The membrane was washed in TBST 2 times for 10 minutes each.
  • 16. Repeated Steps 5-12 with the loading control antibody (for Anti-actin) and its matching secondary antibody.
Anmerkungen - No experimental challenges noted.
Validierungsbilder
Western Blotting validation image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 140-168) antibody (ABIN674709) Figure 1: Western Blot for BAD. Arrowhead indicates the expected molecular weight of ...