Fetuin Antikörper

Produktnummer ABIN99178

Produktdetails anti-Fetuin Antikörper

Target: Fetuin

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Fetuin Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with a recombinant human fetuin (a₂-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This purified polyclonal antibody reacts with human fetuin by ELISA and western blot. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.  A doublet band or slightly lower molecular weight band (see Figure 1, lane 1) may be visible by western blot due to proteolytic processing, variable glycosylation and/or phosphorylation.  Proteolytic processing may include the removal of a 40 amino acid residue bridging peptide from the A and B chains of fetuin in vivo.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu Fetuin

Target: Fetuin

Abstract: Fetuin Produkte

Hintergrund: Human fetuin (2-Heremans-Schmid-glycoprotein or α₂-HS-glycoprotein) is a major plasma glycoprotein predominantly synthesized in the liver.  Human fetuin is named after its bovine homolog.  Fetuins are found in most mammals. Human fetuin is a negative acute-phase protein; normal circulating levels in adults (300–600 μg/ml) fall significantly (30–50%) during injury and infection. The biological role of fetuin is unknown, although it has been implicated as an immunomodulator that can participate in stimulation of bacterial phagocytosis by neutrophils and promotion of endocytosis by mouse macrophages. Hepatocytes are the principal cell source of circulating fetuin, but it also is expressed by monocyte/macrophages. Fetuins occur in large amounts in blood and cerebrospinal fluid and accumulate to high concentrations in calcified bone.  The fetuin promoter region has several potential interleukin 6-responsive elements, and its synthesis is down-regulated during injury and inflammation. Fetuin is an acidic glycoprotein with three N-linked and three O-linked oligosaccharide chains, whose terminal sugar residues are rich in sialic acid (N-acetylneuraminic acid), contributing to its net negative charge. A role for fetuin as a carrier of bioactive molecules has been proposed based on observations that it binds and carries Ca2+ ion.  Fetuin is implicated in bone remodeling, immune function and may play a role in tumor progression of certain cell types.
Synonyms: 59 kDa bone sialic acid-containing protein antibody, A2HS antibody, Aa2-066 antibody, AHS antibody, Ahsg alpha-2-HS-glycoprotein antibody, AHSG antibody, Alpha 2 Z globulin antibody, Alpha-2-HS-glycoprotein antibody

Gen-ID: 197

UniProt: P02765