Maus anti-Human IgG (Heavy & Light Chain) Antikörper

Details zu Produkt Nr. ABIN969481, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
6301
1957
1804
1065
889
727
375
301
35
34
31
15
11
7
3
3
1
1
1
1
1
1
1
Reaktivität
Human
3072
2887
2377
1634
1481
785
690
459
456
397
369
364
301
288
280
146
128
114
42
34
29
26
16
15
11
10
8
7
5
5
4
4
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Wirt
Maus
6987
5101
1927
1047
535
361
90
84
55
35
23
15
8
5
3
1
1
Klonalität (Klon)
Monoklonal ()
Applikation
ELISA, Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Human IgG was isolated from human sera and purified by chromatography.
Klon 4D2D9G8-4A10
Isotyp IgG1
Produktmerkmale Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Reinigung Purified
Antigen
Hintergrund Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Molekulargewicht 50 kDa
Applikations-hinweise Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer PBS containing 0.03 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store at 4 °C or at -20 °C for long term.
Bilder des Herstellers
Western Blotting (WB) image for Mouse anti-Human IgG (Heavy & Light Chain) antibody (ABIN969481) Figure 1: Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb...
Produkt verwendet in: Klonisch, Panayotou, Edwards, Jackson, Berger, Delves, Lund, Roitt: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, 1996 (PubMed).