Maus anti-Human IgG (Heavy & Light Chain) Antikörper

Details zu Produkt Nr. ABIN969481, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
4677
2534
1977
1067
1029
861
306
292
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reaktivität
Human
2883
2746
2299
1668
1446
782
617
444
385
380
351
346
298
297
221
138
135
131
41
31
29
24
16
11
10
8
7
6
5
5
4
4
4
3
3
3
3
3
3
3
2
2
1
1
1
1
1
Wirt
Maus
6779
4841
1792
922
609
372
91
68
55
18
8
5
5
5
2
1
1
Klonalität (Klon)
Monoklonal ()
Applikation
ELISA, Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Human IgG was isolated from human sera and purified by chromatography.
Klon 4D2D9G8-4A10
Isotyp IgG1
Produktmerkmale Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Reinigung Purified
Hintergrund Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Molekulargewicht 50 kDa
Applikationshinweise Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer PBS containing 0.03 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store at 4 °C or at -20 °C for long term.
Bilder des Herstellers
Western Blotting (WB) image for Mouse anti-Human IgG (Heavy & Light Chain) antibody (ABIN969481) Figure 1: Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb...
Produkt verwendet in: Klonisch, Panayotou, Edwards, Jackson, Berger, Delves, Lund, Roitt: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, 1996 (PubMed).