GNAQ Antikörper (AA 22-31)

Produktnummer ABIN968914

Produktdetails anti-GNAQ Antikörper

Target: GNAQ (Guanine Nucleotide Binding Protein (G Protein), Q Polypeptide (GNAQ))

Bindungsspezifität: AA 22-31

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GNAQ Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Maus, Ratte (Rattus), Huhn, Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Galphaq aa. 22-31

Klon: 10-GAQ

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-GNAQ Antikörper (ABIN968914)

Dutt, Kjoller, Giel, Hall, Toksoz: "Activated Galphaq family members induce Rho GTPase activation and Rho-dependent actin filament assembly." in: FEBS letters, Vol. 531, Issue 3, pp. 565-9, (2002) (PubMed).

Li, Iyengar: "Calpain as an effector of the Gq signaling pathway for inhibition of Wnt/beta -catenin-regulated cell proliferation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 20, pp. 13254-9, (2002) (PubMed).

Strathmann, Simon: "G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 23, pp. 9113-7, (1991) (PubMed).

Details zu GNAQ

Target: GNAQ (Guanine Nucleotide Binding Protein (G Protein), Q Polypeptide (GNAQ))

Andere Bezeichnung: Galpha q (GNAQ Produkte)

Hintergrund: The GTP binding regulatory proteins (G proteins) consist of three subunits: alpha, beta, and gamma. These heterotrimeric proteins function at membranes to relay signals from cell surface receptors to intracellular effectors. The alpha subunit is unique for each G protein and contains the site of GTP binding and hydrolysis, as well sites for receptor and effector interactions. The betagamma subunit complex interacts directly with receptors and the alpha subunit. The Galpha family includes four families: the Galphas family including Galphas , Galphao1f, and Galphat, the Galphai family including Galphai, Galphao, and Galphaz, the Galphaq/Galpha11 family and the Galpha12/13 family. The Galphaq protein is 88% homologus with Galpha11 and both are widely expressed. These G proteins activate phospholipase C proteins, which induce calcium signaling events. G protein coupled receptors (GPCRs) involved in regulating Wnt signaling activate Galphaq, phospholipase Cbeta, and induce calcium-dependent activation of calpain. These events promote beta-catenin nuclear export and proteolysis. Galphaq has also been implicated in metabotropic glutamate receptor signaling. Thus, Galphaq isoforms activate phospholipase C proteins in various G-protein coupled receptor pathways.

Molekulargewicht: 42 kDa

Pathways: JAK-STAT Signalweg, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction