UHRF1 Antikörper (AA 199-298)

Produktnummer ABIN968794

Produktdetails anti-UHRF1 Antikörper

Target: UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))

Bindungsspezifität: AA 199-298

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser UHRF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Fluorescence Microscopy (FM)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human ICBP90 aa. 199-298

Klon: 28-ICBP90

Isotyp: IgG2a

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-UHRF1 Antikörper (ABIN968794)

Hopfner, Mousli, Garnier, Redon, du Manoir, Chatton, Ghyselinck, Oudet, Bronner: "Genomic structure and chromosomal mapping of the gene coding for ICBP90, a protein involved in the regulation of the topoisomerase IIalpha gene expression." in: Gene, Vol. 266, Issue 1-2, pp. 15-23, (2001) (PubMed).

Hopfner, Mousli, Jeltsch, Voulgaris, Lutz, Marin, Bellocq, Oudet, Bronner: "ICBP90, a novel human CCAAT binding protein, involved in the regulation of topoisomerase IIalpha expression." in: Cancer research, Vol. 60, Issue 1, pp. 121-8, (2000) (PubMed).

Details zu UHRF1

Target: UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))

Andere Bezeichnung: ICBP90 (UHRF1 Produkte)

Synonyme: ICBP90 antikoerper, Np95 antikoerper, RNF106 antikoerper, hNP95 antikoerper, hUHRF1 antikoerper, AL022808 antikoerper, NP95 antikoerper, fb97f09 antikoerper, wu:fb97f09 antikoerper, zgc:63539 antikoerper, UHRF1 antikoerper, ubiquitin like with PHD and ring finger domains 1 antikoerper, ubiquitin-like, containing PHD and RING finger domains, 1 antikoerper, ubiquitin-like with PHD and ring finger domains 1 antikoerper, UHRF1 antikoerper, Uhrf1 antikoerper, uhrf1 antikoerper

Hintergrund: DNA topoisomerases are ubiquitous nuclear enzymes that alter the topology of DNA. Topoisomerase I produces transient single-stranded breaks in DNA, while topoisomerase II produces transient double-stranded breaks in DNA. These DNA breaks are important for DNA replication and chromosome condensation and segregation. Two types of topoisomerase II have been identified, TopoIIalpha and TopoIIbeta. During interphase, TopoIIalpha is found in the nucleoplasm and nucleoli, and in mitosis, TopoIIalpha is found associated with metaphase chromosomes and centromeres. Transcriptional control of TopoIIalpha expression is regulated through multiple gene promoters, which include five inverted CCAAT boxes, one ATF binding sequence, and two GC-rich boxes. ICBP90 was identified in one-hybrid screen of proteins that can bind an inverted CCAAT box. ICBP90 is expressed highest in proliferative tissues such as thymus, liver, and bone marrow. Overexpression of ICBP90 in COS-1-transfected cells leads to enhanced expression of TopoIIalpha. ICBP90 co-localizes with TopoIIalpha in proliferating areas of appendix and in high grade breast carcinomas. Thus, ICBP90 may be an important transcripitonal regulator of TopoIIalpha expression in proliferating cells.

Molekulargewicht: 97 kDa