VCP Antikörper (AA 9-130)

Produktnummer ABIN968778

Produktdetails anti-VCP Antikörper

Target: VCP (Valosin Containing Protein (VCP))

Bindungsspezifität: AA 9-130

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VCP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Human, Ratte (Rattus), Hund, Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse VCP aa. 9-130

Klon: 18-VCP

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968540, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-VCP Antikörper (ABIN968778)

Zhang, Wang, Kajino, Greene: "VCP, a weak ATPase involved in multiple cellular events, interacts physically with BRCA1 in the nucleus of living cells." in: DNA and cell biology, Vol. 19, Issue 5, pp. 253-63, (2000) (PubMed).

Zhang, Liu, Kobayashi, Tonks: "Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17806-12, (1999) (PubMed).

Zhang, Ashendel, Becker, Morré: "Isolation and characterization of the principal ATPase associated with transitional endoplasmic reticulum of rat liver." in: The Journal of cell biology, Vol. 127, Issue 6 Pt 2, pp. 1871-83, (1995) (PubMed).

Egerton, Ashe, Chen, Druker, Burgess, Samelson: "VCP, the mammalian homolog of cdc48, is tyrosine phosphorylated in response to T cell antigen receptor activation." in: The EMBO journal, Vol. 11, Issue 10, pp. 3533-40, (1992) (PubMed).

Details zu VCP

Target: VCP (Valosin Containing Protein (VCP))

Andere Bezeichnung: VCP (VCP Produkte)

Synonyme: ALS14 antikoerper, IBMPFD antikoerper, TERA antikoerper, p97 antikoerper, CDC48 antikoerper, wu:fd16d05 antikoerper, wu:fj63d11 antikoerper, p93 antikoerper, 3110001E05 antikoerper, p97/VCP antikoerper, 22.26 antikoerper, 7.12 antikoerper, Apple antikoerper, BcDNA.GM02885 antikoerper, BcDNA:GM02885 antikoerper, CG2331 antikoerper, DmTER94 antikoerper, Dmel\CG2331 antikoerper, I antikoerper, Ter94 antikoerper, Tera antikoerper, VCP antikoerper, VCP ATPase antikoerper, VCP/p97 antikoerper, Vcp antikoerper, anon-WO2004063362.65 antikoerper, anon-WO2004063362.67 antikoerper, cdc48 antikoerper, dTERA antikoerper, dVCP antikoerper, dmTERA antikoerper, dvcp antikoerper, l(2)03775 antikoerper, l(2)46CFf antikoerper, l(2)46CFs antikoerper, l(2)46Ch antikoerper, pTER94 antikoerper, ter94 antikoerper, vcp antikoerper, FANCG antikoerper, valosin containing protein antikoerper, valosin containing protein S homeolog antikoerper, valosin-containing protein antikoerper, CG2331 gene product from transcript CG2331-RA antikoerper, zgc:136908 antikoerper, transitional endoplasmic reticulum ATPase antikoerper, VCP antikoerper, vcp.S antikoerper, vcp antikoerper, Vcp antikoerper, Bm1_56585 antikoerper, LOC100136246 antikoerper, TER94 antikoerper, zgc:136908 antikoerper, LOC101109680 antikoerper

Hintergrund: ATPases that belong to the ATPase associated with different cellular activities (AAA) family are homo-oligomeric proteins that have roles in cell cycle regulation, protein degradation, organelle biogenesis, and vesicle mediated-protein transport. Valosin containing protein (VCP) is a AAA family member that is found as a hexamer in rat liver. In vitro, VCP catalyzes the hydrolysis of ATP, but this activity is distinct from classical transport ATPases. VCP protein is found primarily in the transitional elements between the rough and smooth ER (TER), but stimulation with EGF leads to VCP translocation to the nucleus. Antibodies to VCP can perturb cell-free formation of transition vesicles from isolated TER of rat liver, and can inhibit transfer of material from ER to Golgi in a reconstituted membrane system. VCP is phosphorylated after T-cell activation, and PTPH1 dephosphorylation of VCP correlates with suppression of cell growth. In addition, the N-terminal region of VCP binds to the DNA damage repair protein, BRCA1. Thus, VCP may have roles in both ER protein transport and nuclear function, which are important for cell growth and survival. This antibody is routinely tested by western blot analysis.
Synonyms: Valosin Containing Protein

Molekulargewicht: 97 kDa

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway