GBF1 Antikörper (AA 1266-1379)

Produktnummer ABIN968745

Produktdetails anti-GBF1 Antikörper

Target: GBF1 (Golgi Brefeldin A Resistant Guanine Nucleotide Exchange Factor 1 (GBF1))

Bindungsspezifität: AA 1266-1379

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GBF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human GBF1 aa. 1266-1379

Klon: 25-GBF1

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968535, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-GBF1 Antikörper (ABIN968745)

Claude, Zhao, Kuziemsky, Dahan, Berger, Yan, Armold, Sullivan, Melançon: "GBF1: A novel Golgi-associated BFA-resistant guanine nucleotide exchange factor that displays specificity for ADP-ribosylation factor 5." in: The Journal of cell biology, Vol. 146, Issue 1, pp. 71-84, (1999) (PubMed).

Mansour, Herbrick, Scherer, Melançon: "Human GBF1 is a ubiquitously expressed gene of the sec7 domain family mapping to 10q24." in: Genomics, Vol. 54, Issue 2, pp. 323-7, (1999) (PubMed).

Details zu GBF1

Target: GBF1 (Golgi Brefeldin A Resistant Guanine Nucleotide Exchange Factor 1 (GBF1))

Andere Bezeichnung: GBF1 (GBF1 Produkte)

Synonyme: ARF1GEF antikoerper, MGC148393 antikoerper, DKFZp459D2411 antikoerper, arf1gef antikoerper, 1700083E03Rik antikoerper, AI035702 antikoerper, AW049900 antikoerper, mKIAA0248 antikoerper, RGD1307160 antikoerper, golgi brefeldin A resistant guanine nucleotide exchange factor 1 antikoerper, Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 antikoerper, golgi-specific brefeldin A-resistance factor 1 antikoerper, GBF1 antikoerper, LOC100185387 antikoerper, gbf1 antikoerper, Gbf1 antikoerper

Hintergrund: The Golgi apparatus is a complex and dynamic organelle that functions in protein sorting and modification. Numerous structural and regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles. Golgi-specific Brefeldin A (BFA)-resistance Factor (GBF1) is a guanine nucleotide exchange factor that contains a centrally located Sec7 domain, and a proline-rich region at the C-terminus. The Sec7 domain is commonly found in a variety of secretory proteins, and is required for activation of ARFs. GBF1 mRNA is ubiquitously expressed, and GBF1 protein localizes to the Golgi, as well as to perinuclear structures that contain COPI. BFA is a fungal heterocyclic lactone that disrupts membrane recruitment of ARFs. This action blocks protein secretion, and changes the morphology of various organelles, such as the Golgi. Overexpression of GBF1 suppresses BFA-induced changes in Golgi morphology, ARF activation, and coat protein recruitment. GBF1 interacts preferentially with ARF5 in the presence of Mg2+. Thus, GBF1 is a Golgi-specific guanine nucleotide exchange factor that activates ARFs through a mechanism that interferes with BFA action.
Synonyms: Golgi-specific Brefeldin A (BFA)-resistance Factor 1

Molekulargewicht: 206 kDa