XRCC4 Antikörper (AA 53-168)

Produktnummer ABIN968566

Produktdetails anti-XRCC4 Antikörper

Target: XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))

Bindungsspezifität: AA 53-168

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser XRCC4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human XRCC4 aa. 53-168

Klon: 4-XRCC4

Isotyp: IgG2b

Anwendungsinformationen

Kommentare:

Related Products: ABIN968535, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-XRCC4 Antikörper (ABIN968566)

Gao, Sun, Frank, Dikkes, Fujiwara, Seidl, Sekiguchi, Rathbun, Swat, Wang, Bronson, Malynn, Bryans, Zhu, Chaudhuri, Davidson, Ferrini, Stamato, Orkin, Greenberg, Alt: "A critical role for DNA end-joining proteins in both lymphogenesis and neurogenesis." in: Cell, Vol. 95, Issue 7, pp. 891-902, (1999) (PubMed).

Modesti, Hesse, Gellert: "DNA binding of Xrcc4 protein is associated with V(D)J recombination but not with stimulation of DNA ligase IV activity." in: The EMBO journal, Vol. 18, Issue 7, pp. 2008-18, (1999) (PubMed).

Li, Otevrel, Gao, Cheng, Seed, Stamato, Taccioli, Alt: "The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination." in: Cell, Vol. 83, Issue 7, pp. 1079-89, (1996) (PubMed).

Details zu XRCC4

Target: XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))

Andere Bezeichnung: XRCC4 (XRCC4 Produkte)

Synonyme: zgc:73312 antikoerper, MGC81443 antikoerper, homolog of human DNA ligase iv-binding protein XRCC4 antikoerper, 2310057B22Rik antikoerper, AW413319 antikoerper, AW545101 antikoerper, X-ray repair cross complementing 4 antikoerper, X-ray repair complementing defective repair in Chinese hamster cells 4 antikoerper, X-ray repair complementing defective repair in Chinese hamster cells 4 L homeolog antikoerper, DNA ligase IV-binding protein antikoerper, XRCC4 antikoerper, xrcc4 antikoerper, xrcc4.L antikoerper, Xrcc4 antikoerper

Hintergrund: DNA double-strand breaks (DSB) are generated during intrinsic eukaryotic DNA recombination events such as assembly of antigen receptor genes and meiotic and mitotic recombination. DSB repair proteins are also required to repair breaks induced by extrinsic factors such as ionizing radiation and mutagenic chemicals. DNA-PKcs, Ku70/Ku80, DNA ligase IV, and X-Ray Cross Complementation group 4 (XRCC4) are DSB proteins involved in both V(D)J recombination and DNA double-stranded break repair. XRCC4 activates DNA ligase IV and cells deficient in XRCC4 inefficiently form coding joints and signal joints during V(D)J recombination. XRCC4 contains a C-terminal nuclear localization sequence (NLS) and multiple phosphorylation sites, binds DNA, and is an effective substrate for DNA-PK. Phosphorylation of XRCC4 has no effect on its interactions with DNA ligase IV or end-joining activity, but can inhibit its DNA binding activity. Mice deficient in XRCC4 exhibit defects in lymphogenesis and apoptotic death of postmitotic neurons during neurogenesis. Thus, XRCC4 is a ubiquitous protein involved in DNA end joining during DNA recombination and repair, which is critical for cell growth and survival.
Synonyms: X-Ray Cross Complementation group 4

Molekulargewicht: 55 kDa

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response