EPS15 Antikörper (AA 694-888)
-
- Target Alle EPS15 Antikörper anzeigen
- EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))
-
Bindungsspezifität
- AA 694-888
-
Reaktivität
- Human, Maus, Ratte
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser EPS15 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
- Kreuzreaktivität
- Maus, Ratte (Rattus)
- Produktmerkmale
-
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - Aufreinigung
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Human Eps15 aa. 694-888
- Klon
- 17-eps15
- Isotyp
- IgG2a
- Top Product
- Discover our top product EPS15 Primärantikörper
-
-
- Kommentare
-
Related Products: ABIN968537, ABIN967389
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store undiluted at -20° C.
-
-
The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro." in: The Journal of cell biology, Vol. 154, Issue 6, pp. 1209-23, (2001) (PubMed).
: "Regulation of complex formation of POB1/epsin/adaptor protein complex 2 by mitotic phosphorylation." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18399-406, (2000) (PubMed).
: "Crystal structure of the alpha appendage of AP-2 reveals a recruitment platform for clathrin-coat assembly." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 16, pp. 8907-12, (1999) (PubMed).
: "Mapping of the molecular determinants involved in the interaction between eps15 and AP-2." in: Cancer research, Vol. 57, Issue 2, pp. 240-5, (1997) (PubMed).
: "Structural requirements of the epidermal growth factor receptor for tyrosine phosphorylation of eps8 and eps15, substrates lacking Src SH2 homology domains." in: The Journal of biological chemistry, Vol. 270, Issue 27, pp. 16271-6, (1995) (PubMed).
: "
-
The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro." in: The Journal of cell biology, Vol. 154, Issue 6, pp. 1209-23, (2001) (PubMed).
-
- Target
- EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))
- Andere Bezeichnung
- Eps15 (EPS15 Produkte)
- Synonyme
- AF-1P antikoerper, AF1P antikoerper, MLLT5 antikoerper, CG16932 antikoerper, Dmel\\CG16932 antikoerper, EH1 antikoerper, EPS-15 antikoerper, Eps15 antikoerper, anon-WO0118547.85 antikoerper, eps-15 antikoerper, eps15 antikoerper, i31 antikoerper, 2410112D09Rik antikoerper, RGD1307641 antikoerper, epidermal growth factor receptor pathway substrate 15 antikoerper, Epidermal growth factor receptor pathway substrate clone 15 antikoerper, hypothetical protein antikoerper, EPS15 antikoerper, Eps-15 antikoerper, eps15 antikoerper, Eps15 antikoerper, PGTG_06574 antikoerper
- Hintergrund
- The eps15 (EGF receptor pathway substrate clone number 15) gene was isolated using an expression cloning method designed for direct isolation of cDNAs encoding substrates of tyrosine kinases. The eps15 gene encodes a 145 kDa protein that is tyrosine phosphorylated following EGF receptor activation and this receptor directly phosphorylates purified Eps15 in vitro. Phosphorylation of Eps15 is much more efficient following EGF receptor activation versus erbB2 kinase activation, suggesting the protein is predominantly a part of an EGF receptor signaling pathway. Overexpression of Eps15 causes transformation of NIH 3T3 cells, implying Eps15 may be involved in the control of cell proliferation. Eps15 binds to the SH3 domain of Crk through its proline-rich domain at the C-terminal region. Adaptin alpha binds to the C-terminus of Eps15 at three different sites ranging from residues 650 to 730. Eps15, Adaptin alpha, and EGF receptor are co-localized at the clathrin-coated pits after receptor activation. Therefore, Eps15 may recruit EGF receptors to the clathrin-coated pits for internalization of the activated receptor. This antibody is routinely tested by western blot analysis.
- Molekulargewicht
- 145 kDa
- Pathways
- EGFR Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, EGFR Downregulation
-