SMARCA2 Antikörper (AA 1400-1586)

Produktnummer ABIN967939

Produktdetails anti-SMARCA2 Antikörper

Target: SMARCA2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))

Bindungsspezifität: AA 1400-1586

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SMARCA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Kreuzreaktivität: Maus, Ratte (Rattus), Hund, Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Brm aa. 1400-1586

Klon: 24-BRM

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968535, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-SMARCA2 Antikörper (ABIN967939)

Wong, Shanahan, Chen, Lian, Ha, Hendricks, Ghaffari, Iliev, Penn, Woodland, Smith, Salada, Carillo, Laity, Gupte, Swedlund, Tavtigian, Teng, Lees: "BRG1, a component of the SWI-SNF complex, is mutated in multiple human tumor cell lines." in: Cancer research, Vol. 60, Issue 21, pp. 6171-7, (2000) (PubMed).

Singh, Coe, Hong: "A role for retinoblastoma protein in potentiating transcriptional activation by the glucocorticoid receptor." in: Nature, Vol. 374, Issue 6522, pp. 562-5, (1995) (PubMed).

Muchardt, Yaniv: "A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor." in: The EMBO journal, Vol. 12, Issue 11, pp. 4279-90, (1993) (PubMed).

Details zu SMARCA2

Target: SMARCA2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))

Andere Bezeichnung: Brm (SMARCA2 Produkte)

Synonyme: BAF190 antikoerper, BRM antikoerper, NCBRS antikoerper, SNF2 antikoerper, SNF2L2 antikoerper, SNF2LA antikoerper, SWI2 antikoerper, Sth1p antikoerper, hBRM antikoerper, hSNF2a antikoerper, brm antikoerper, 2610209L14Rik antikoerper, SNF2alpha antikoerper, Snf2l2 antikoerper, brahma antikoerper, psa-4 antikoerper, wu:fa56c07 antikoerper, wu:fi27f11 antikoerper, zgc:66238 antikoerper, Brahma antikoerper, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 antikoerper, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 L homeolog antikoerper, SMARCA2 antikoerper, smarca2.L antikoerper, Smarca2 antikoerper, smarca2 antikoerper

Hintergrund: Several of the SNF and SWI genes of Saccharomyces cerevisiae encode proteins that are involved in the regulation of transcriptional activation. One of these proteins, SNF2/SWI2, has both Drosophila and mammalian homologues, designated brm. The human Brm protein has been reported to be a 180 kDa nuclear factor that acts as a transcriptional activator when fused to a heterologus DNA binding domain. Transfected Brm, expressed in cells lacking endogenous protein, can cooperate with the glucocorticoid receptor (GR) in transcriptional activation. The cooperation between Brm and GR requires the DNA binding domain of GR and the helicase domain and the P/Q-charged domain of Brm. However, Brm does not affect on several other transcription factors. The retinoblastoma protein, Rb, stimulates the transcription of a number of genes. Like Brm, Rb up-regulates glucocorticoid-receptor-mediated transcription. Brm and Rb interact in vitro and in vivo, requiring the Rb-pocket domain and the consensus Rb-binding motif of Brm. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 180 kDa