SERPINB5 Antikörper

Produktnummer ABIN967456

Produktdetails anti-SERPINB5 Antikörper

Target: SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SERPINB5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Human Maspin

Klon: G167-70

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Applications include western blot analysis (1-2 µg/ml). A-431 human epidermal carcinoma cells (ATCC CRL 1555) are recommended as a positive control for this application.

Kommentare:

Related Products: ABIN967389, ABIN968533

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-SERPINB5 Antikörper (ABIN967456)

Sheng, Pemberton, Sager: "Production, purification, and characterization of recombinant maspin proteins." in: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 30988-93, (1995) (PubMed).

Hopkins, Whisstock: "Function of maspin." in: Science (New York, N.Y.), Vol. 265, Issue 5180, pp. 1893-4, (1994) (PubMed).

Zou, Anisowicz, Hendrix, Thor, Neveu, Sheng, Rafidi, Seftor, Sager: "Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells." in: Science (New York, N.Y.), Vol. 263, Issue 5146, pp. 526-9, (1994) (PubMed).

Details zu SERPINB5

Target: SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))

Andere Bezeichnung: Maspin (SERPINB5 Produkte)

Synonyme: 1110036M19Rik antikoerper, AI462524 antikoerper, AI646751 antikoerper, Maspin antikoerper, PI-5 antikoerper, Spi7 antikoerper, ovalbumin antikoerper, PI5 antikoerper, maspin antikoerper, Pi5 antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 5 antikoerper, serpin family B member 5 antikoerper, serpin peptidase inhibitor, clade B (ovalbumin), member 5 S homeolog antikoerper, Serpinb5 antikoerper, SERPINB5 antikoerper, serpinb5.S antikoerper

Hintergrund: Maspin is a 42 kDa protein that may play a role in human breast cancer by functioning as a tumor suppressor. Maspin is expressed in normal mammary epithelial cells, but there is a loss of expression in invasive breast cancer including lymph node and distant metastases. Further, maspin is not expressed in most mammary carcinoma cell lines. Mammary carcinoma cells transfected with recombinant maspin have a reduced ability to migrate through reconstituted basement membrane and lose their ability to form tumors in nude mice, providing support for maspin as a tumor suppressor protein. Maspin has homology to members of the serpin family of protease inhibitors, some of which have been implicated in the regulation of invasion and metastasis. Yet protease inhibitory activity has not been detected using recombinant maspin. It has been suggested that maspin, unlike most serpins, may be a ligand-binding serpin rather than a protease inhibitor. However, the question of whether or not maspin has protease inhibitor activity remains to be determined. G167-70 is specific for human maspin. It detects maspin as a 42 kD protein in western blots of normal human mammary epithelial cells. Many mammary tumor cell lines including MDA-MB-231 (ATCC HTB 26) and MDA-MB-435S (ATCC HTB 129) lack maspin expression and are suitable as negative controls. Purified recombinant human maspin from a yeast expression system was used as immunogen.

Molekulargewicht: 42 kDa

Pathways: p53 Signalweg