DCC Antikörper (Deleted in Colorectal Carcinoma) (Extracellular Domain)

Details for Product anti-DCC Antibody No. ABIN967442, Anbieter: Anmelden zum Anzeigen
Antigen
  • CRC18
  • CRCR1
  • IGDCC1
  • MRMV1
  • DCC
  • zdcc
  • C030036D22Rik
  • Igdcc1
  • xdcc
  • XDCCa
  • dcca-A
  • Dcc
  • DCC netrin 1 receptor
  • deleted in colorectal carcinoma
  • netrin receptor DCC
  • DCC
  • dcc
  • Dcc
  • LOC100712666
Epitop
Extracellular Domain
11
11
10
10
10
9
8
5
3
3
2
2
2
2
1
1
Reaktivität
Human
62
54
21
4
4
4
3
3
2
2
2
1
1
Wirt
Maus
73
11
6
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser DCC Antikörper ist unkonjugiert
5
5
4
4
4
4
Applikation
Flow Cytometry (FACS), Western Blotting (WB)
65
42
21
13
8
3
3
1
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Marke BD Pharmingen™
Immunogen Recombinant Human DCC (extracellular domain)
Klon G92-13
Isotyp IgG1
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung DCC (DCC Antibody Abstract)
Hintergrund One of the most common regions of allelic loss in colorectal tumors is chromosome 18, which is lost in more than 70% of carcinomas, and in almost 50% of late adenomas. This region of loss has been mapped to chromosome 18q and a gene called Deleted in Colorectal Cancer (DCC). DCC encodes an ~185 kD glycoprotein with significant homology to the neural cell adhesion molecule and other related cell surface glycoproteins. The predicted amino acid sequence of DCC cDNA consists of a 1448 amino acid (aa) long transmembrane phosphoprotein. The extracellular domain consists of 1098 amino acids and has 42% sequence homology to cell adhesion proteins of the neural cell adhesion molecule (N-CAM) family. DCC mRNA is found to be expressed in normal colonic mucosa, but its expression is reduced or absent in the majority of colorectal carcinomas. The loss of heterozygosity and subsequent alteration of DCC expression has also been observed in tumors of non-colorectal origin. G92-13 recognizes human DCC. A truncated recombinant protein containing the extracellular domain of the human DCC was used as immunogen.
Synonyms: Deleted in Colon Cancer
Molekulargewicht 175-190 kDa
Pathways Regulation of Cell Size
Applikationshinweise IMR 32 human neuroblastoma cells (ATCC CCL 127) are suggested as a positive control. By western blot, DCC-specific antibodies typically identify protein species with molecular weights of ~175-190 kD. Doublets in this range have been reported in brain. Several smaller immunoreactive species, representing degradation products, cross-reactive species, or DCC forms arising from alternative splicing of DCC mRNA or in vivo processing of the DCC protein may also be identified. Another DCC clone (mAb G97-449, ABIN967443) has been published for immunohistochemical staining of paraffin-embedded tissue sections.
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Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C.
Bilder des Herstellers
Western Blotting (WB) image for anti-Deleted in Colorectal Carcinoma (DCC) (Extracellular Domain) antibody (ABIN967442) Western blot analysis of human DCC protein in 293 human embryonic kidney cells stably...
 image for anti-Deleted in Colorectal Carcinoma (DCC) (Extracellular Domain) antibody (ABIN967442) anti-Deleted in Colorectal Carcinoma (DCC) (Extracellular Domain) antibody (Image 2)
Produkt verwendet in: Shibata, Reale, Lavin, Silverman, Fearon, Steele, Jessup, Loda, Summerhayes: "The DCC protein and prognosis in colorectal cancer." in: The New England journal of medicine, Vol. 335, Issue 23, pp. 1727-32, 1996 (PubMed).

Reale, Hu, Zafar, Getzenberg, Levine, Fearon: "Expression and alternative splicing of the deleted in colorectal cancer (DCC) gene in normal and malignant tissues." in: Cancer research, Vol. 54, Issue 16, pp. 4493-501, 1994 (PubMed).

Fearon, Cho, Nigro, Kern, Simons, Ruppert, Hamilton, Preisinger, Thomas, Kinzler: "Identification of a chromosome 18q gene that is altered in colorectal cancers." in: Science (New York, N.Y.), Vol. 247, Issue 4938, pp. 49-56, 1990 (PubMed).

Vogelstein, Fearon, Hamilton, Kern, Preisinger, Leppert, Nakamura, White, Smits, Bos: "Genetic alterations during colorectal-tumor development." in: The New England journal of medicine, Vol. 319, Issue 9, pp. 525-32, 1988 (PubMed).

Fearon, Hamilton, Vogelstein: "Clonal analysis of human colorectal tumors." in: Science (New York, N.Y.), Vol. 238, Issue 4824, pp. 193-7, 1987 (PubMed).

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