BCL2 Antikörper (B-Cell CLL/lymphoma 2) (AA 41-54)

Details for Product anti-BCL2 Antibody No. ABIN967321, Anbieter: Anmelden zum Anzeigen
Antigen
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • BCL2, apoptosis regulator
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2a
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • bcl2a
  • BCL-2
Epitop
AA 41-54
164
77
66
64
44
31
25
25
24
21
18
15
15
15
13
13
13
11
9
9
7
6
6
5
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
1066
266
220
54
28
22
18
18
5
4
4
3
2
2
2
2
Wirt
Maus
579
553
18
6
2
2
2
Klonalität
Monoklonal
Konjugat
Dieser BCL2 Antikörper ist unkonjugiert
53
50
45
41
35
25
24
16
15
15
14
14
14
14
14
14
13
13
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)
853
398
396
343
338
327
231
196
170
81
42
15
14
13
12
10
9
4
3
2
1
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Marke BD Pharmingen™
Immunogen Human Bcl-2 synthetic peptide aa. 41-54
Isotyp IgG1
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung Bcl-2 (BCL2 Antibody Abstract)
Hintergrund Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types. Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation. Because Bcl-2 blocks apoptosis it may contribute to tumorigenisis by prolonging cell survival, rather than by accelerating the rate of cell proliferation. Human Bcl-2 protein migrates at a molecular weight of ~26 kDa by SDS-PAGE. Bcl-2/100 recognizes a 26 kDa band representing human Bcl-2. Additional minor bands at 27-31 kDa and 18-21 kDa may also be visualized. The 27-31 kDa upper band may represent a larger isoform, whereas the 18-21 kDa lower band may be an internal translation or proteolytic product, therefore, a synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG) of human Bcl-2 was used as immunogen. This peptide sequence is not conserved between human and mouse. Bcl-2/100 does not cross-react with mouse Bcl-2. For detection of mouse Bcl-2 refer to clone 3F11 (ABIN967441), polyclonal rabbit anti-rat/mouse Bcl-2 antiserum, and polyclonal rabbit anti-mouse Bcl-2 antiserum.
Pathways MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
Applikationshinweise Western Blot: Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Store lysate at -20° C). Additional control lysate (ABIN968537) is sold separately.
IHC: For paraffin-embedded sections, tissues should be fixed in either Bouin's fixative or formalin. For immunofluorescent staining and flow cytometry, our directly conjugated formats are recommended.
Kommentare

Related Products: ABIN968537

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.25 mg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20°C.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (ABIN967321) Profile of permeabilized lymphocytes analyzed on a FACScan (BDIS, San Jose, CA) (Firs...
Western Blotting (WB) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (ABIN967321) Western blot analysis of Bcl-2 (Second Panel). Lysate from Jurkat cells was probed wi...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (ABIN967321) Formalin-fixed, paraffin-embedded tissue sections stained for Bcl-2 expression using ...
Produkt verwendet in: Krajewski, Tanaka, Takayama, Schibler, Fenton, Reed: "Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes." in: Cancer research, Vol. 53, Issue 19, pp. 4701-14, 1993 (PubMed).

Pezzella, Turley, Kuzu, Tungekar, Dunnill, Pierce, Harris, Gatter, Mason: "bcl-2 protein in non-small-cell lung carcinoma." in: The New England journal of medicine, Vol. 329, Issue 10, pp. 690-4, 1993 (PubMed).

Tanaka, Saito, Reed: "Structure-function analysis of the Bcl-2 oncoprotein. Addition of a heterologous transmembrane domain to portions of the Bcl-2 beta protein restores function as a regulator of cell survival." in: The Journal of biological chemistry, Vol. 268, Issue 15, pp. 10920-6, 1993 (PubMed).

Pezzella, Jones, Ralfkiaer, Ersbøll, Gatter, Mason: "Evaluation of bcl-2 protein expression and 14;18 translocation as prognostic markers in follicular lymphoma." in: British journal of cancer, Vol. 65, Issue 1, pp. 87-9, 1992 (PubMed).

Reed, Meister, Tanaka, Cuddy, Yum, Geyer, Pleasure: "Differential expression of bcl2 protooncogene in neuroblastoma and other human tumor cell lines of neural origin." in: Cancer research, Vol. 51, Issue 24, pp. 6529-38, 1992 (PubMed).

Williams: "Programmed cell death: apoptosis and oncogenesis." in: Cell, Vol. 65, Issue 7, pp. 1097-8, 1991 (PubMed).

Pezzella, Tse, Cordell, Pulford, Gatter, Mason: "Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation." in: The American journal of pathology, Vol. 137, Issue 2, pp. 225-32, 1990 (PubMed).

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