VAV1 Antikörper (Vav 1 Oncogene) (pTyr174)

Details for Product anti-VAV1 Antibody No. ABIN967241
Antigen
  • VAV
  • Vav
  • vav-T
  • vav guanine nucleotide exchange factor 1
  • vav 1 oncogene
  • vav guanine nucleotide exchange factor 1 L homeolog
  • VAV1
  • Vav1
  • vav1.L
Epitop
pTyr174
42
17
14
9
9
8
7
6
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
163
86
71
3
3
2
1
1
1
1
Wirt
Kaninchen
149
16
Konjugat
Dieser VAV1 Antikörper ist unkonjugiert
6
4
4
4
4
4
4
2
2
2
2
2
Applikation
Western Blotting (WB)
125
68
32
12
7
6
6
5
2
1
1
Optionen
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Vav around the phosphorylation site of tyrosine 174 (E-I-YP-E-D).
Spezifität Vav (phospho-Tyr174) antibody detects endogenous levels of Vav only when phosphorylated at tyrosine 174.
Reinigung The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Plasmids, Primers & others Plasmide, Primers & weitere VAV1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung Vav (VAV1 Antibody Abstract)
Pathways T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, CXCR4-mediated Signaling Events
Applikations-hinweise WB: 1:500~1:1000.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Allgemeine Veröffentlichungen Amarasinghe, Rosen: "Acidic region tyrosines provide access points for allosteric activation of the autoinhibited Vav1 Dbl homology domain." in: Biochemistry, Vol. 44, Issue 46, pp. 15257-68, 2005 (PubMed).

Miura-Shimura, Duan, Rao, Reddi, Shimura, Rottapel, Druker, Tsygankov, Band, Band: "Cbl-mediated ubiquitinylation and negative regulation of Vav." in: The Journal of biological chemistry, Vol. 278, Issue 40, pp. 38495-504, 2003 (PubMed).

Produkt verwendet in: Charvet, Canonigo, Billadeau, Altman: "Membrane localization and function of Vav3 in T cells depend on its association with the adapter SLP-76." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 15289-99, 2005 (PubMed).

Kuhne, Ku, Weiss: "A guanine nucleotide exchange factor-independent function of Vav1 in transcriptional activation." in: The Journal of biological chemistry, Vol. 275, Issue 3, pp. 2185-90, 2000 (PubMed).

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