p34 Cdc-2protein (Nuclear) Antikörper

Produktnummer ABIN966737

Produktdetails

Target: p34 Cdc-2protein

Bindungsspezifität: Nuclear

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: This antibody reacts with a 34 kD cyclic dependent serine/threonine kinase protein. P34 cdc2 and cyclin B1 are catalytic subunits of the maturation promoting factor (MPF). This antigen is activated with cyclins through dephosphrylation of tyrosine residue, initiates the entry of cells into mitosis, and is followed by the breakdown of the nuclear envelope. Expression of this antigen reflects the proliferating potential of positively staining tissue.

Aufreinigung: Concentrated.

Immunogen: The recombinant human p34.cdc2 fusion protein.

Klon: B322 (POH-1)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Antigendetails

Target: p34 Cdc-2protein

Gen-ID: 983