p105 Proliferating-Associated Nuclear Antigen (Nuclear) Antikörper

Produktnummer ABIN966725

Produktdetails

Target: p105 Proliferating-Associated Nuclear Antigen

Bindungsspezifität: Nuclear

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)

Spezifität: The p105 protein, found in all human cells in a dimeric form (105 kD) and monomeric form (41 kD), is a proliferation-associated nuclear antigen that is absent in non-cycling cells in the G0 phase. This protein is important in the synthesis and transport of RNA in the cell regulation cycle. During G2 and mitosis, this protein is increased possibly due to the unmasking of nucleolar antigens, resulting in large accumulations of this protein in the mitotic cytoplasm.

Aufreinigung: Concentrated.

Immunogen: Activated peripheral blood nuclei.

Klon: B301

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1:50-1:100. Formalin-fixed paraffin-embedded (FFPE) tissue sections require pepsin or trypsin digestion.
Western blotting: 1:100-1:200 .
Staining Procedure: This antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. FFPE tissue sections require pepsin or trypsin digestion. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.

Kommentare:

Cellular Localization: Nuclear.
Recommended Positive Control: Human tonsil or lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Antigendetails

Target: p105 Proliferating-Associated Nuclear Antigen

Molekulargewicht: Dimeric form 105 kDa, Monomeric form 41 kDa

Gen-ID: 4790