Western Bloting: 1/500 - 1/2000. ELISA: Propose dilution 1/10000. Not yet tested in other applications. Determining optimal working dilutions by titration test.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Sommer, Stadtfeld, Murphy, Hochedlinger, Kotton, Mostoslavsky: "Induced pluripotent stem cell generation using a single lentiviral stem cell cassette." in: Stem cells (Dayton, Ohio), Vol. 27, Issue 3, pp. 543-9, (2009) (PubMed).
Ettwiller, Budd, Spitz, Wittbrodt: "Analysis of mammalian gene batteries reveals both stable ancestral cores and highly dynamic regulatory sequences." in: Genome biology, Vol. 9, Issue 12, pp. R172, (2009) (PubMed).
Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in proteinprotein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation. Oct4 is renowned as a transcription factor expressed by undifferentiated embryonic stem cells and embryonic germ cells. Synonyms: OCT3, POU5F1