XRCC6 Antikörper (AA 506-541)

Produktnummer ABIN966469

Produktdetails anti-XRCC6 Antikörper

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Bindungsspezifität: AA 506-541

Reaktivität: Human, Maus, Affe, Xenopus laevis, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser XRCC6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Aufreinigung: Concentrated. Purified from the ascites fluid by Protein A chromatography

Immunogen: Human placental extract designated as PSE1-PL

Klon: N3H10

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunofluorescence
Immunoprecipitation (Native and denatured)(Use Protein A) (Ab 2µg/mg protein lysate)
Western blotting: (0.25-0.5 µg/ml for 2hrs at RT)
Immunohistology (Formalinparaffin)(Ab 1-2g/ml for 30 minute at RT)
Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH 6.0.

Kommentare:

Cellular Localization: Nuclear.
Recommended Positive Control: BT474, HEP-G-2 or HeLa Cells, Tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL.

Buffer: 10mM PBS, pH 7.4, with 0.2% BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-XRCC6 Antikörper (ABIN966469)

Wang, Chou, Blankson, Satoh, Knuth, Eisenberg, Pisetsky, Reeves: "Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens." in: Molecular biology reports, Vol. 18, Issue 1, pp. 15-28, (1993) (PubMed).

Details zu XRCC6

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Andere Bezeichnung: Ku (p70) (XRCC6 Produkte)

Synonyme: CTC75 antikoerper, CTCBF antikoerper, G22P1 antikoerper, KU70 antikoerper, ML8 antikoerper, TLAA antikoerper, 70kDa antikoerper, G22p1 antikoerper, Ku70 antikoerper, Kup70 antikoerper, X-ray repair cross complementing 6 antikoerper, ATP-dependent DNA helicase II, 70 kDa subunit antikoerper, X-ray repair complementing defective repair in Chinese hamster cells 6 L homeolog antikoerper, X-ray repair complementing defective repair in Chinese hamster cells 6 antikoerper, XRCC6 antikoerper, Bm1_41430 antikoerper, xrcc6.L antikoerper, Xrcc6 antikoerper

Hintergrund: The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Also available without BSA and azide at 1mg/ml.

Molekulargewicht: 70kDa

Gen-ID: 2547

Pathways: DNA Reparatur