AKT1
Reaktivität: Human
WB, IHC, IF, IP
Wirt: Kaninchen
Monoclonal
unconjugated
Applikationshinweise
Western Bloting: 1/500 - 1/2000. ELISA: Propose dilution 1/10000. Not yet tested in other applications. Determining optimal working dilutions by titration test.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Zhao, Chen, Cai, Yang, Sui, Fu: "Angiotensin II/angiotensin II type I receptor (AT1R) signaling promotes MCF-7 breast cancer cells survival via PI3-kinase/Akt pathway." in: Journal of cellular physiology, Vol. 225, Issue 1, pp. 168-73, (2010) (PubMed).
DSouza-Schorey, Boshans, McDonough, Stahl, Van Aelst: "A role for POR1, a Rac1-interacting protein, in ARF6-mediated cytoskeletal rearrangements." in: The EMBO journal, Vol. 16, Issue 17, pp. 5445-54, (1997) (PubMed).
Datta, Franke, Chan, Makris, Yang, Kaplan, Morrison, Golemis, Tsichlis: "AH/PH domain-mediated interaction between Akt molecules and its potential role in Akt regulation." in: Molecular and cellular biology, Vol. 15, Issue 4, pp. 2304-10, (1995) (PubMed).
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. Synonyms: AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, AKT1