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Kaninchen anti-Maus IgG (Heavy & Light Chain) Antikörper - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
Produktnummer ABIN965192
  • Target Alle IgG Produkte
    IgG
    Fragment
    F(ab')2 fragment
    Bindungsspezifität
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reaktivität
    • 694
    • 672
    • 561
    • 458
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 97
    • 95
    • 92
    • 62
    • 47
    • 38
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 1730
    • 1473
    • 539
    • 206
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Kaninchen
    Klonalität
    • 3360
    • 167
    • 1
    Polyklonal
    Applikation
    • 1839
    • 1800
    • 1791
    • 1500
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 52
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Spezifität
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.
    Produktmerkmale
    F(ab')2 MOUSE IgG (H&L) Antibody Pre-Adsorbed was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 MOUSE IgG (H&L) Antibody Pre-Adsorbed is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
    Aufreinigung
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.

    Immunogen Type: Native Protein

    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

    ELISA Dilution: 1:2,000 - 1:20,000

    Western Blot Dilution: 1:1,000 - 1:10,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store secondary antibody conjugate at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: Rabbit F(ab')2 Anti-Mouse IgG Antibody Pre-Adsorbed

    Background: F(ab')2 MOUSE IgG (H&L) Antibody Pre-Adsorbed was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 MOUSE IgG (H&L) Antibody Pre-Adsorbed is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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