Ziege anti-Ratte (Rattus) IgG (Heavy & Light Chain) Antikörper (Atto 488)

Details zu Produkt Nr. ABIN964999, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
6231
2030
1776
1135
782
648
372
305
34
34
31
15
12
12
12
12
11
7
3
3
1
1
1
1
1
1
1
Reaktivität
Ratte (Rattus)
3080
2913
2382
1661
1509
785
737
489
476
410
401
395
341
298
271
160
159
111
54
34
30
26
16
15
12
10
8
7
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Wirt
Ziege
7129
5285
1972
1077
507
362
89
84
51
35
23
15
8
5
3
1
1
Klonalität
Polyklonal
Konjugat
Atto 488
2227
1820
1751
1266
793
717
430
343
235
147
145
140
140
116
113
112
102
98
94
93
92
91
89
84
81
80
78
75
73
72
68
68
65
61
55
54
54
54
54
53
53
53
53
53
53
52
48
43
41
37
32
31
23
23
22
20
19
18
17
16
15
15
15
14
14
14
12
12
12
12
12
11
11
10
10
10
10
9
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
Immunoassay (IA)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Rat IgG whole molecule
Isotyp IgG
Spezifität Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.
Produktmerkmale Anti-Rat IgG (H&L) conjugated to ATTO 488 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reinigung This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Antigen
Applikations-hinweise Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light.
Lagerung -20 °C
Informationen zur Lagerung Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 488) (ABIN964999) Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 488)
Immunofluorescence (IF) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 488) (ABIN964999) Immunofluorescence of anti rat IgG antibody Tissue: Normal mouse spleen Fixation: met...
Immunofluorescence (IF) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 488) (ABIN964999) Immunofluorescence of anti rat IgG antibody Tissue: Normal mouse spleen Fixation: use...
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Atto 488) (ABIN964999) Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb...