Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (Atto 594)

Details zu Produkt Nr. ABIN964989
Antigen
Epitop
Heavy & Light Chain
840
233
200
38
13
8
Reaktivität
Kaninchen
222
197
165
143
124
76
75
57
53
53
44
41
40
35
23
14
13
7
7
7
3
2
Wirt
Ziege
575
499
185
44
36
32
7
7
1
Klonalität
Polyklonal
Konjugat
Atto 594
181
161
138
131
129
127
46
35
32
29
28
24
16
11
9
9
9
9
7
6
5
5
5
5
5
5
5
5
4
4
4
4
2
2
2
2
1
1
1
1
Applikation
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Optionen
Immunogen

Immunogen: Rabbit IgG whole molecule

Isotyp IgG
Spezifität Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Kreuzreaktivität (Details) Pre-Adsorbed
Produktmerkmale Anti-Rabbit IgG (H&L) conjugated to ATTO 594 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reinigung Rabbit IgG (H&L) Antibody ATTO 594 was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.  This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
Labeling Ratio 2.5
Antigen
Hintergrund

Synonyms: Goat anti-Rabbit IgG Antibody ATTO594 Conjugation, Goat anti-Rabbit IgG ATTO 594 Conjugated Antibody

Background: Anti-Rabbit IgG (H&L) ATTO 594 Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Applikations-hinweise

Application Note: Anti-Rabbit IgG (H&L) conjugated to ATTO 594 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Kommentare

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
Lagerung RT,4 °C,-20 °C
Informationen zur Lagerung Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 594) (ABIN964989) Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs,...
 image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 594) (ABIN964989) Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 594) (Image 2)
Produkt verwendet in: Kummer, Avinoam, Kräusslich: "IFITM3 Clusters on Virus Containing Endosomes and Lysosomes Early in the Influenza A Infection of Human Airway Epithelial Cells." in: Viruses, Vol. 11, Issue 6, 2019 (PubMed).

Coffman, Kachur, Pilgrim, Dawes: "Antagonistic Behaviors of NMY-1 and NMY-2 Maintain Ring Channels in the C. elegans Gonad." in: Biophysical journal, Vol. 111, Issue 10, pp. 2202-2213, 2017 (PubMed).